Production of landomycins in Streptomyces globisporus 1912 and S cyanogenus S136 is regulated by genes encoding putative transcriptional activators

被引：47

作者：

Rebets, Y

Ostash, B

Luzhetskyy, A

Hoffmeister, D

Brana, A

Mendez, C

Salas, JA

Bechtold, A

Fedorenko, V

机构：

[1] Ivan Franko Natl Univ Lviv, Dept Genet & Biotechnol, UA-79005 Lvov, Ukraine

[2] Univ Freiburg, D-79104 Freiburg, Germany

[3] Univ Oviedo, Dept Biol Funcional, Oviedo 33006, Spain

[4] Univ Oviedo, Inst Univ Oncol Principado Asturias, Oviedo 33006, Spain

来源：

FEMS MICROBIOLOGY LETTERS | 2003年 / 222卷 / 01期

关键词：

landomycin; regulation; angucycline; Streptomyces;

D O I：

10.1016/S0378-1097(03)00258-1

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The regulatory genes lanI and IndI have been cloned from the landomycin A (LaA) producer Streptomyces cyanogenus S136 and from the landomycin E (LaE) producer Streptomyces globisporus 1912, respectively and both have been sequenced. A culture of S. globisporus I2-1 carrying a disrupted IndI gene did not produce LaE and other related intermediates. Complementation of S. globisporus I2-1 with either the lndI or lanI gene reconstituted LaE production indicating that LanI and Lndl are involved in activation of structural genes in the respective clusters. Structural features of these regulatory genes are discussed. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：149 / 153

页数：5

共 19 条

[1] PLASMID CLONING VECTORS FOR THE CONJUGAL TRANSFER OF DNA FROM ESCHERICHIA-COLI TO STREPTOMYCES SPP [J].