Closing and characterization of an endogenous COS-7 cell thromboxane A2 receptor

被引：10

作者：

Becker, KP

Ullian, M

Halushka, PV

机构：

[1] Med Univ S Carolina, Dept Cell & Mol Pharmacol & Expt Therapeut, Div Clin Pharmacol, Charleston, SC 29425 USA

[2] Med Univ S Carolina, Dept Med, Div Clin Pharmacol, Charleston, SC 29425 USA

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH | 1998年 / 1403卷 / 01期

关键词：

thromboxane A(2) receptor; cDNA; cloning; kidney cell; monkey;

D O I：

10.1016/S0167-4889(98)00019-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A cDNA for a thromboxane A(2) (TXA(2)) receptor was cloned from an SV40 transformed African Green Monkey kidney cell line (COS-7). The sequence is 98% homologous with the isoform of the human TXA(2) receptor and has agonist and antagonist ligand binding characteristics that are not significantly different from the human receptor. Stimulation of the COS-7 cells with the TXA(2) receptor agonist, ONO 11113 resulted in a significant increase in cAMP formation that was blocked by a receptor antagonist. The results raise the question of the utility of the COS-7 cell line for studies of cloned and expressed TXA(2) receptor signalling mechanisms. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：109 / 114

页数：6

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