Phasing the 30S ribosomal subunit structure

被引：12

作者：

Brodersen, DE ^{[1
]}

Clemons, WM ^{[1
]}

Carter, AP ^{[1
]}

Wimberly, BT ^{[1
]}

Ramakrishnan, V ^{[1
]}

机构：

[1] MRC, Mol Biol Lab, Cambridge CB2 2QH, England

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 2003年 / 59卷

关键词：

D O I：

10.1107/S0907444903017669

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The methods involved in determining the 850 kDa structure of the 30S ribosomal subunit from Thermus thermophilus were in many ways identical to those that are generally used in standard protein crystallography. This paper reviews and analyses the methods that can be used in phasing such large structures and shows that the anomalous signal collected from heavy-atom compounds bound to the RNA is both necessary and sufficient for ab initio structure determination at high resolution. In addition, measures to counter problems with non-isomorphism and radiation decay are described.

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页码：2044 / 2050

页数：7

相关论文

共 19 条

[1] Methods used in the structure determination of bovine mitochondrial F-1 ATPase [J].

Abrahams, JP ;

Leslie, AGW .

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 1996, 52 :30-42

[2] THE CCP4 SUITE - PROGRAMS FOR PROTEIN CRYSTALLOGRAPHY [J].

BAILEY, S .

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 1994, 50 :760-763

[3] The complete atomic structure of the large ribosomal subunit at 2.4 Å resolution [J].

Ban, N ;

Nissen, P ;

Hansen, J ;

Moore, PB ;

Steitz, TA .

SCIENCE, 2000, 289 (5481) :905-920

[4] A 9Å resolution x-ray crystallographic map of the large ribosomal subunit [J].

Ban, N ;

Freeborn, B ;

Nissen, P ;

Penczek, P ;

Grassucci, RA ;

Sweet, R ;

Frank, J ;

Moore, PB ;

Steitz, TA .

CELL, 1998, 93 (07) :1105-1115

[5]

Blundell T. L., 1976, Protein crystallography

[6] X-ray crystal structures of 70S ribosome functional complexes [J].

Cate, JH ;

Yusupov, MM ;

Yusupova, GZ ;

Earnest, TN ;

Noller, HF .

SCIENCE, 1999, 285 (5436) :2095-2104

[7] Construction of low-resolution X-ray crystallographic electron density maps of the ribosome [J].

Cate, JHD .

METHODS, 2001, 25 (03) :303-308

[8] Crystal structure of the 30 S ribosomal subunit from Thermus thermophilus:: Purification, crystallization and structure determination [J].

Clemons, WM ;

Brodersen, DE ;

McCutcheon, JP ;

May, JLC ;

Carter, AP ;

Morgan-Warren, RJ ;

Wimberly, BT ;

Ramakrishnan, V .

JOURNAL OF MOLECULAR BIOLOGY, 2001, 310 (04) :827-843

[9] Structure of a bacterial 30S ribosomal subunit at 5.5 Å resolution [J].

Clemons, WM ;

May, JLC ;

Wimberly, BT ;

McCutcheon, JP ;

Capel, MS ;

Ramakrishnan, V .

NATURE, 1999, 400 (6747) :833-840

[10] Density modification for macromolecular phase improvement [J].

Cowtan, KD ;

Zhang, KYJ .

PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY, 1999, 72 (03) :245-270