Influence of the shape of phospholipid vesicles on the measurement of their size by photon correlation spectroscopy

被引：16

作者：

Beney, L ^{[1
]}

Linares, E ^{[1
]}

Ferret, E ^{[1
]}

Gervais, P ^{[1
]}

机构：

[1] Univ Bourgogne 1, Ecole Natl Super Biol Appl Nutr & Alimentat, Lab Genie Procedes Alimentaires & Biotechnol, F-21000 Dijon, France

来源：

EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS | 1998年 / 27卷 / 06期

关键词：

liposomes; shape; diffusion coefficient; photon correlation spectroscopy;

D O I：

10.1007/s002490050167

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

The influence of shape transformation of large unilamellar vesicles (LUV) on their size measurement by photon correlation spectroscopy (PCS) has been investigated. The experimental size of vesicles after hyperosmotic contractions of increasing intensities have been compared to the theoretical volume decrease determined by applying Boyle Van't Hoff's law. The main observation is that PCS size measurement gives overestimated values when LUV have been subjected to a volume decrease of more than 20% of their initial volume. The PCS size overestimation is related to the influence of the shape transformation of the vesicles on their diffusion coefficient (D) as shown by modelling the evolution of D of a sphere which is transformed into an ellipsoid by internal volume reduction under constant area.

引用

页码：567 / 574

页数：8

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