Analysis of 5-methylcytosine in DNA of breast and colon cancer tissues

被引：18

作者：

Barciszewska, Anna-Maria ^{[2
]}

Murawa, Dawid ^{[3
]}

Gawronska, Iwona ^{[1
]}

Murawa, Pawel ^{[3
]}

Nowak, Stanislaw ^{[2
]}

Barciszewska, Miroslawa Z. ^{[1
]}

机构：

[1] Polish Acad Sci, Inst Bioorgan Chem, PL-61704 Poznan, Poland

[2] Poznan Univ Med Sci, Dept & Clin Neurosurg & Neurotraumatol, Poznan, Poland

[3] Wielkopolska Ctr Oncol, Ward Surg Oncol 1, Poznan, Poland

来源：

IUBMB LIFE | 2007年 / 59卷 / 12期

关键词：

DNA methylation; m(5)C analysis; epigenetics; breast cancer; colon cancer; diagnostics;

D O I：

10.1080/15216540701697412

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

5-methylcytosine (m(5)C) can be used as a sensitive marker of progress of the tumor formation induced by the oxidative damage reactions. We have analyzed the amount of m(5)C in DNA of patients with breast and colon cancers. Two dimensional thin layer chromatography (TLC) has been used to monitor 5-methylcytosine level in DNA extracted from cancer tissues. The level of methylation of cytosine at C-5 position in DNA from breast cancer patients correlates well with the malignancy of tumors. Interestingly higher amount of m(5)C in DNA for the breast cancer patients treated with different chemotherapeutics was observed. It suggests an activation of DNA methyltransferase as well as a genomic suppression of the DNA repair genes expression. These differences clearly reflect the health condition of patients and support the global analysis of m(5)C in DNA as a good marker for diagnosis of neoplasia in clinical practice.

引用

页码：765 / 770

页数：6

共 37 条

[1] Human and bacterial oxidative demethylases repair alkylation damage in both RNA and DNA [J].

Aas, PA ;

Otterlei, M ;

Falnes, PO ;

Vågbo, CB ;

Skorpen, F ;

Akbari, M ;

Sundheim, O ;

Bjorås, M ;

Slupphaug, G ;

Seeberg, E ;

Krokan, HE .

NATURE, 2003, 421 (6925) :859-863

[2] Aberrant patterns of DNA methylation, chromatin formation and gene expression in cancer [J].

Baylin, SB ;

Esteller, M ;

Rountree, MR ;

Bachman, KE ;

Schuebel, K ;

Herman, JG .

HUMAN MOLECULAR GENETICS, 2001, 10 (07) :687-692

[3] Epidemiology of colorectal cancer [J].

Boyle, P ;

Leon, ME .

BRITISH MEDICAL BULLETIN, 2002, 64 :1-25

[4] DNA methylation: Bisulphite modification and analysis [J].

Clark, Susan J. ;

Statham, Aaron ;

Stirzaker, Clare ;

Molloy, Peter L. ;

Frommer, Marianne .

NATURE PROTOCOLS, 2006, 1 (05) :2353-2364

[5] Hypomethylation: One side of a larger picture [J].

Dunn, BK .

EPIGENETICS IN CANCER PREVENTION: EARLY DETECTION AND RISK ASSESSMENT, 2003, 983 :28-42

[6] DNA hypomethylation, cancer, the immunodeficiency, centromeric region instability, facial anomalies syndrome and chromosomal rearrangements [J].

Ehrlich, M .

JOURNAL OF NUTRITION, 2002, 132 (08) :2424S-2429S

[7] DNA methylation in cancer: too much, but also too little [J].

Ehrlich, M .

ONCOGENE, 2002, 21 (35) :5400-5413

[8] Expression of various genes is controlled by DNA methylation during mammalian development [J].

Ehrlich, M .

JOURNAL OF CELLULAR BIOCHEMISTRY, 2003, 88 (05) :899-910

[9] Hypomethylation and hypermethylation of DNA in Wilms tumors [J].

Ehrlich, M ;

Jiang, GC ;

Fiala, E ;

Dome, JS ;

Yu, MC ;

Long, TI ;

Youn, B ;

Sohn, OS ;

Widschwendter, M ;

Tomlinson, GE ;

Chintagumpala, M ;

Champagne, M ;

Parham, D ;

Liang, GN ;

Malik, K ;

Laird, PW .

ONCOGENE, 2002, 21 (43) :6694-6702

[10] AlkB-mediated oxidative demethylation reverses DNA damage in Escherichia coli [J].

Falnes, PO ;

Johansen, RF ;

Seeberg, E .

NATURE, 2002, 419 (6903) :178-182

← 1 2 3 4 →