Cooling rates during flash cooling

被引：64

作者：

Teng, TY ^{[1
]}

Moffat, K ^{[1
]}

机构：

[1] Univ Chicago, Dept Biochem & Mol Biol, Chicago, IL 60637 USA

来源：

JOURNAL OF APPLIED CRYSTALLOGRAPHY | 1998年 / 31卷

关键词：

D O I：

10.1107/S0021889897009229

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Flash cooling of macromolecular crystals was investigated by measurement of the cooling rates achieved by different cryoprotectant solutions (glycerol, sucrose and polyethylene glycol) with different sample volumes (0.2-0.8mm(3)) and by different cooling agents (cold nitrogen or helium gas, liquid nitrogen and liquid propane). Samples were not cooled instantaneously; it usually took 0.5 to 1 s to reach the glass transition temperature and 1 to 2 s to reach the final temperature. The cooling rate varied from similar to 50 to 700 K s(-1) depending on the volume of the sample, the cooling agent used and the temperature at the time of measurement. The cooling rate may affect the distribution of unit-cell dimensions, structural states and the average structure throughout the volume of the crystal, and hence its mosaic spread.

引用

页码：252 / 257

页数：6

共 21 条

[1]

BALD WB, 1974, J EXP BOT, V26, P103

[2] STRUCTURE OF A B-DNA DODECAMER AT 16-K [J].

DREW, HR ;

SAMSON, S ;

DICKERSON, RE .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1982, 79 (13) :4040-4044

[3] THERMAL-EXPANSION OF A PROTEIN [J].

FRAUENFELDER, H ;

HARTMANN, H ;

KARPLUS, M ;

KUNTZ, ID ;

KURIYAN, J ;

PARAK, F ;

PETSKO, GA ;

RINGE, D ;

TILTON, RF ;

CONNOLLY, ML ;

MAX, N .

BIOCHEMISTRY, 1987, 26 (01) :254-261

[4] TEMPERATURE-DEPENDENT X-RAY-DIFFRACTION AS A PROBE OF PROTEIN STRUCTURAL DYNAMICS [J].

FRAUENFELDER, H ;

PETSKO, GA ;

TSERNOGLOU, D .

NATURE, 1979, 280 (5723) :558-563

[5] HIGH RESOLUTION (1.05Å) STRUCTURE OF SALMONELLA TYPHIMURIUM NEURAMINIDASE (SIALIDASE) [J].

Garman, E. ;

Wouters, J. ;

Vimr, E. ;

Laver, G. ;

Sheldrick, G. M. .

ACTA CRYSTALLOGRAPHICA A-FOUNDATION AND ADVANCES, 1996, 52 :C8-C8

[6] Glycerol concentrations required for cryoprotection of 50 typical protein crystallization solutions [J].

Garman, EF ;

Mitchell, EP .

JOURNAL OF APPLIED CRYSTALLOGRAPHY, 1996, 29 :584-587

[7] Macromolecular cryocrystallography [J].

Garman, EF ;

Schneider, TR .

JOURNAL OF APPLIED CRYSTALLOGRAPHY, 1997, 30 :211-237

[8] CONFORMATIONAL SUBSTATES IN A PROTEIN - STRUCTURE AND DYNAMICS OF METMYOGLOBIN AT 80-K [J].

HARTMANN, H ;

PARAK, F ;

STEIGEMANN, W ;

PETSKO, GA ;

PONZI, DR ;

FRAUENFELDER, H .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1982, 79 (16) :4967-4971

[9] CRYOCRYSTALLOGRAPHY OF BIOLOGICAL MACROMOLECULES - A GENERALLY APPLICABLE METHOD [J].

HOPE, H .

ACTA CRYSTALLOGRAPHICA SECTION B-STRUCTURAL SCIENCE, 1988, 44 :22-26

[10]

HOPE H, 1990, ANNU REV BIOPHYS BIO, V19, P107

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