Atmospheric pressure photoionization of peptides

被引：12

作者：

Bagag, A. ^{[2
]}

Giuliani, A. ^{[1
,3
]}

Laprevote, O. ^{[2
,4
]}

机构：

[1] Synchrotron SOLEIL, DISCO Beamline, Orme Merisiers, F-91192 Gif Sur Yvette, France

[2] CNRS, Lab Spectrometrie Masse, ICSN, F-91190 Gif Sur Yvette, France

[3] INRA, Cepia, F-44316 Nantes 3, France

[4] Univ Paris 05, Lab ChimToxicol Analyt & Cellulaire, EA 4463, Fac Sci Pharmaceut & Biol, F-75006 Paris, France

来源：

INTERNATIONAL JOURNAL OF MASS SPECTROMETRY | 2011年 / 299卷 / 01期

关键词：

Atmospheric pressure photoionization; Peptide fragmentation; ECD/ETD; ELECTRON-CAPTURE DISSOCIATION; TANDEM MASS-SPECTROMETRY; TOP-DOWN; PROTEIN IONS; SEQUENCE TAGS; IDENTIFICATION; FRAGMENTATION; MECHANISM;

D O I：

10.1016/j.ijms.2010.08.010

中图分类号：

O64 [物理化学（理论化学）、化学物理学]; O56 [分子物理学、原子物理学];

学科分类号：

070305 [高分子化学与物理];

摘要：

In this work, fragmentations of peptides under atmospheric pressure photoionization conditions are further investigated for a model peptide. In the positive ion mode, intensive fragmentations are observed, mainly ascribed to c-sequence ions along with less abundant a- and b- type ions. The same features and c- ion series are reported for the negative ion mode, thereby suggesting a common mechanistic origin for the fragmentation in both ion modes. Moreover, it is shown that ECD-like fragmentation under APPI conditions may retain non-covalent adducts. This interesting property positions APPI as a promising ECD-like technique for in-source top down sequencing. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：1 / 4

页数：4

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