Fluorescence nanoscopy in whole cells by asynchronous localization of photoswitching emitters

被引:190
作者
Egner, Alexander [1 ]
Geisler, Claudia
Von Middendorff, Claas
Bock, Hannes
Wenzel, Dirk
Medda, Rebecca
Andresen, Martin
Stiel, Andre C.
Jakobs, Stefan
Eggeling, Christian
Schoenle, Andreas
Hell, Stefan W.
机构
[1] Max Planck Inst Biophys Chem, Dept NanoBiophoton, Gottingen, Germany
[2] Max Planck Inst Biophys Chem, Dept Neurobiol, D-37077 Gottingen, Germany
关键词
D O I
10.1529/biophysj.107.112201
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
We demonstrate nanoscale resolution in far-field fluorescence microscopy using reversible photoswitching and localization of individual fluorophores at comparatively fast recording speeds and from the interior of intact cells. These advancements have become possible by asynchronously recording the photon bursts of individual molecular switching cycles. We present images from the microtubular network of an intact mammalian cell with a resolution of 40 nm.
引用
收藏
页码:3285 / 3290
页数:6
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