Fluorescence nanoscopy in whole cells by asynchronous localization of photoswitching emitters

被引：189

作者：

Egner, Alexander ^{[1
]}

Geisler, Claudia

Von Middendorff, Claas

Bock, Hannes

Wenzel, Dirk

Medda, Rebecca

Andresen, Martin

Stiel, Andre C.

Jakobs, Stefan

Eggeling, Christian

Schoenle, Andreas

Hell, Stefan W.

机构：

[1] Max Planck Inst Biophys Chem, Dept NanoBiophoton, Gottingen, Germany

[2] Max Planck Inst Biophys Chem, Dept Neurobiol, D-37077 Gottingen, Germany

来源：

BIOPHYSICAL JOURNAL | 2007年 / 93卷 / 09期

关键词：

D O I：

10.1529/biophysj.107.112201

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

We demonstrate nanoscale resolution in far-field fluorescence microscopy using reversible photoswitching and localization of individual fluorophores at comparatively fast recording speeds and from the interior of intact cells. These advancements have become possible by asynchronously recording the photon bursts of individual molecular switching cycles. We present images from the microtubular network of an intact mammalian cell with a resolution of 40 nm.

引用

页码：3285 / 3290

页数：6

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