Nonlinear and asymmetric open channel characteristics of an ion-selective porin in planar membranes

被引：20

作者：

Mathes, A ^{[1
]}

Engelhardt, H ^{[1
]}

机构：

[1] Max Planck Inst Biochem, Abt Mol Strukturbiol, D-82152 Martinsried, Germany

来源：

BIOPHYSICAL JOURNAL | 1998年 / 75卷 / 03期

关键词：

D O I：

10.1016/S0006-3495(98)74045-7

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

The open channel characteristics of the bacterial porin Omp32 from Comamonas acidovorans were investigated by means of conductance measurements in planar lipid bilayers of the Montal-Mueller type. Particularly at low salt conditions (less than or equal to 30 mM KCI) Omp32 exhibited some unusual asymmetric and nonlinear functional properties. Current-voltage relationship measurements showed that conductance depends on the orientation of porin molecules and is a nonlinear function of the applied membrane potential. Conductance also depends on the salt concentration in a manner not common to porins and the salt concentration modulates the nonlinearity of conductance-voltage relationships. Omp32 is strongly anion-selective. The nonlinear and asymmetric conductance of the open channel is a new observation in porins.

引用

页码：1255 / 1262

页数：8

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