Differential expression of sonic hedgehog immunoreactivity during lesion evolution in autoimmune encephalomyelitis

被引:41
作者
Seifert, T
Bauer, J
Weissert, R
Fazekas, F
Storch, MK
机构
[1] Med Univ, Div Neuroimmunol, Vienna, Austria
[2] Univ Tubingen, Hertie Inst Clin Brain Res, Dept Gen Neurol, Tubingen, Germany
关键词
experimental autoimmune encephalomyelitis; multiple sclerosis; myelin oligodendrocyte glycoprotein; remyelination; sonic hedgehog;
D O I
10.1093/jnen/64.5.404
中图分类号
R74 [神经病学与精神病学];
学科分类号
摘要
The signaling molecule Sonic hedgehog (Shh) is involved in several processes of central nervous system development. Recent reports indicate that Shh expression plays a role also in certain pathologic conditions in the adult brain, including multiple sclerosis and its animal model. However, the role of Shh signaling in immune-mediated demyelinating disease remains still uncertain. The aim of our study was to investigate the distribution pattern of Shh immunoreactivity (Shh-IR) during lesion evolution in myelin-oligodendrocyte-glycoprotein-induced experimental autoimmune encephalomyelitis (MOG-EAE), a model strongly mimicking multiple sclerosis. MOG-EAE was actively induced in DA rats. Histologic evaluation was performed with light and confocal microscopy on paraffin-embedded central nervous system sections from days 20 to 120 after active immunization. Shh-IR was present within the lesions of MOG-EAE during all stages of lesion evolution. The highest staining intensity for Shh was found in remyelinating lesions. In actively demyelinating, inactive demyelinated lesions, and in remyelinating lesions, Shh-IR was detected in macrophages, endothelium, and astrocytes. Shh-IR in axons was exclusively present in remyelinating lesions. Although the exact molecular mechanisms of the Shh-signaling pathway in experimental autoimmune encephalomyelitis are yet to be determined, our findings may imply a role of Shh signaling in facilitating remyelination.
引用
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页码:404 / 411
页数:8
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