Population structure of nuclear and mitochondrial DNA variation among humpback whales in the North Pacific

被引:96
作者
Baker, CS
Medrano-Gonzalez, L
Calambokidis, J
Perry, A
Pichler, F
Rosenbaum, H
Straley, JM
Urban-Ramirez, J
Yamaguchi, M
Von Ziegesar, O
机构
[1] Univ Auckland, Sch Biol Sci, Auckland 1, New Zealand
[2] Univ Nacl Autonoma Mexico, Fac Ciencias, Mexico City 04510, DF, Mexico
[3] Cascadia Res Collect, Olympia, WA 98501 USA
[4] Amer Museum Nat Hist, New York, NY 10024 USA
[5] Univ Alaska SE, Sitka, AK 99835 USA
[6] Univ Autonoma Baja California Sur, Dept Biol Marina, La Paz 23081, BCS, Mexico
[7] Ogasawara Marine Ctr, Ogasawara, Tokyo 10021, Japan
[8] N Gulf Ocean Soc, Homer, AK 99603 USA
关键词
control region; intron; microsatellite; management; stocks; gene flow;
D O I
10.1046/j.1365-294x.1998.00384.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The population structure of variation in a nuclear actin intron and the control region of mitochondrial DNA is described for humpback whales from eight regions in the North Pacific Ocean: central California, Baja Peninsula, nearshore Mexico (Bahia Banderas), offshore Mexico (Socorro Island), southeastern Alaska, central Alaska (Prince Williams Sound), Hawaii and Japan (Ogasawara Islands). Primary mtDNA haplotypes and intron alleles were identified using selected restriction fragment length polymorphisms of target sequences amplified by the polymerase chain reaction (PCR-RFLP). There was little evidence of heterogeneity in the frequencies of mtDNA haplotypes or actin intron alleles due to the year or sex composition of the sample. However, frequencies of four mtlDNA haplotypes showed marked regional differences in their distributions (Phi(ST) = 0.277; P < 0.001; n = 205 individuals) while the two alleles showed significant, but less marked, regional differences (Phi(ST) = 0.033; P < 0.013; n = 400 chromosomes). An hierarchical analysis of variance in frequencies of haplotypes and alleles supported the grouping of six regions into a central and eastern stock with further partitioning of variance among regions within stocks for haplotypes but not for alleles. Based on available genetic and demographic evidence, the southeastern Alaska and central California feeding grounds were selected for additional analyses of nuclear differentiation using allelic variation at four microsatellite loci. All four loci showed significant differences in allele frequencies (overall F-ST = 0.043; P < 0.001; average n = 139 chromosomes per locus), indicating at least partial reproductive isolation between the two regions as well as the segregation of mtDNA lineages. Although the two feeding grounds were not panmictic for nuclear or mitochondrial loci, estimates of long-term migration rates suggested that male-mediated gene flow was several-fold greater than female gene flow. These results include and extend the range and sample size of previously published work, providing additional evidence for the significance of genetic management units within oceanic populations of humpback whales.
引用
收藏
页码:695 / 707
页数:13
相关论文
共 72 条
[1]  
Amato G., 1994, Experientia Supplementum (Basel), V69, P215
[2]  
[Anonymous], B FAR SEAS FISH RES
[3]  
Avise J. C., 1994, NATURAL HIST GENETIC
[4]   POPULATION CHARACTERISTICS OF DNA FINGERPRINTS IN HUMPBACK WHALES (MEGAPTERA-NOVAEANGLIAE) [J].
BAKER, CS ;
GILBERT, DA ;
WEINRICH, MT ;
LAMBERTSEN, R ;
CALAMBOKIDIS, J ;
MCARDLE, B ;
CHAMBERS, GK ;
OBRIEN, SJ .
JOURNAL OF HEREDITY, 1993, 84 (04) :281-290
[5]   MIGRATORY MOVEMENT AND POPULATION-STRUCTURE OF HUMPBACK WHALES (MEGAPTERA-NOVAEANGLIAE) IN THE CENTRAL AND EASTERN NORTH PACIFIC [J].
BAKER, CS ;
HERMAN, LM ;
PERRY, A ;
LAWTON, WS ;
STRALEY, JM ;
WOLMAN, AA ;
KAUFMAN, GD ;
WINN, HE ;
HALL, JD ;
REINKE, JM ;
OSTMAN, J .
MARINE ECOLOGY PROGRESS SERIES, 1986, 31 (02) :105-119
[6]  
BAKER CS, 1992, FISH B-NOAA, V90, P429
[7]   AGGRESSIVE-BEHAVIOR BETWEEN HUMPBACK WHALES (MEGAPTERA-NOVAEANGLIAE) WINTERING IN HAWAIIAN WATERS [J].
BAKER, CS ;
HERMAN, LM .
CANADIAN JOURNAL OF ZOOLOGY, 1984, 62 (10) :1922-1937
[8]   ABUNDANT MITOCHONDRIAL-DNA VARIATION AND WORLDWIDE POPULATION-STRUCTURE IN HUMPBACK WHALES [J].
BAKER, CS ;
PERRY, A ;
BANNISTER, JL ;
WEINRICH, MT ;
ABERNETHY, RB ;
CALAMBOKIDIS, J ;
LIEN, J ;
LAMBERTSEN, RH ;
RAMIREZ, JU ;
VASQUEZ, O ;
CLAPHAM, PJ ;
ALLING, A ;
OBRIEN, SJ ;
PALUMBI, SR .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (17) :8239-8243
[9]   ALTERNATIVE POPULATION ESTIMATES OF HUMPBACK WHALES (MEGAPTERA-NOVAEANGLIAE) IN HAWAIIAN WATERS [J].
BAKER, CS ;
HERMAN, LM .
CANADIAN JOURNAL OF ZOOLOGY, 1987, 65 (11) :2818-2821
[10]   INFLUENCE OF SEASONAL MIGRATION ON GEOGRAPHIC-DISTRIBUTION OF MITOCHONDRIAL-DNA HAPLOTYPES IN HUMPBACK WHALES [J].
BAKER, CS ;
PALUMBI, SR ;
LAMBERTSEN, RH ;
WEINRICH, MT ;
CALAMBOKIDIS, J ;
OBRIEN, SJ .
NATURE, 1990, 344 (6263) :238-240