Human gut Bacteroidetes can utilize yeast mannan through a selfish mechanism

被引：463

作者：

Cuskin, Fiona ^{[1
,2
]}

Lowe, Elisabeth C. ^{[1
]}

Temple, Max J. ^{[1
]}

Zhu, Yanping ^{[1
,2
]}

Cameron, Elizabeth A. ^{[3
]}

Pudlo, Nicholas A. ^{[3
]}

Porter, Nathan T. ^{[3
]}

Urs, Karthik ^{[3
]}

Thompson, Andrew J. ^{[4
]}

Cartmell, Alan ^{[5
,6
]}

Rogowski, Artur ^{[1
]}

Hamilton, Brian S. ^{[7
]}

Chen, Rui ^{[8
]}

Tolbert, Thomas J. ^{[9
]}

Piens, Kathleen ^{[10
]}

Bracke, Debby ^{[10
]}

Vervecken, Wouter ^{[10
]}

Hakki, Zalihe ^{[5
,6
]}

Speciale, Gaetano ^{[5
,6
]}

Munoz-Munoz, Jose L. ^{[1
]}

Day, Andrew ^{[1
]}

Pena, Maria J. ^{[2
]}

McLean, Richard ^{[11
]}

Suits, Michael D. ^{[12
]}

Boraston, Alisdair B. ^{[12
]}

Atherly, Todd ^{[13
]}

Ziemer, Cherie J. ^{[13
]}

Williams, Spencer J. ^{[5
,6
]}

Davies, Gideon J. ^{[4
]}

Abbott, D. Wade ^{[2
,11
]}

Martens, Eric C. ^{[3
]}

Gilbert, Harry J. ^{[1
,2
]}

机构：

[1] Newcastle Univ, Inst Cell & Mol Biosci, Newcastle Upon Tyne NE2 4HH, Tyne & Wear, England

[2] Univ Georgia, Complex Carbohydrate Res Ctr, Athens, GA 30602 USA

[3] Univ Michigan, Sch Med, Dept Microbiol & Immunol, Ann Arbor, MI 48109 USA

[4] Univ York, Dept Chem, York YO10 5DD, N Yorkshire, England

[5] Univ Melbourne, Sch Chem, Parkville, Vic 3010, Australia

[6] Univ Melbourne, Mol Sci & Biotechnol Inst Bio21, Parkville, Vic 3010, Australia

[7] Indiana Univ, Interdisciplinary Biochem Grad Program, Bloomington, IN 47405 USA

[8] Indiana Univ, Dept Chem, Bloomington, IN 47405 USA

[9] Univ Kansas, Sch Pharm, Dept Pharmaceut Chem, Lawrence, KS 66047 USA

[10] Oxyrane, B-9052 Ghent, Belgium

[11] Agr & Agrifood Canada, Lethbridge Res Ctr, Lethbridge, AB T1J 4B1, Canada

[12] Univ Victoria, Victoria, BC V8P 5C2, Canada

[13] ARS, USDA, Natl Lab Agr & Environm, Ames, IA 50011 USA

来源：

NATURE | 2015年 / 517卷 / 7533期

基金：

英国生物技术与生命科学研究理事会; 欧洲研究理事会; 澳大利亚研究理事会; 英国惠康基金; 美国国家卫生研究院;

关键词：

CARBOHYDRATE-ACTIVE ENZYMES; POLYSACCHARIDE UTILIZATION; BACTERIA; CATABOLISM; MICROBIOTA; INSIGHTS; COMPLEX; FAMILY; STARCH;

D O I：

10.1038/nature13995

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

Yeasts, which have been a component of the human diet for at least 7,000 years, possess an elaborate cell wall alpha-mannan. The influence of yeast mannan on the ecology of the human microbiota is unknown. Here we show that yeast alpha-mannan is a viable food source for the Gram-negative bacterium Bacteroides thetaiotaomicron, a dominant member of the microbiota. Detailed biochemical analysis and targeted gene disruption studies support a model whereby limited cleavage of alpha-mannan on the surface generates large oligosaccharides that are subsequently depolymerized to mannose by the action of periplasmic enzymes. Co-culturing studies showed that metabolism of yeast mannan by B. thetaiotaomicron presents a 'selfish' model for the catabolism of this difficult to breakdown polysaccharide. Genomic comparison with B. thetaiotaomicron in conjunction with cell culture studies show that a cohort of highly successful members of the microbiota has evolved to consume sterically-restricted yeast glycans, an adaptation that may reflect the incorporation of eukaryotic microorganisms into the human diet.

引用

页码：165 / U86

页数：21

共 42 条

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A COMPREHENSIVE PROCEDURE FOR PREPARATION OF PARTIALLY METHYLATED ALDITOL ACETATES FROM GLYCOPROTEIN CARBOHYDRATES [J].