Haematological, biochemical and toxicological investigations in spontaneous cases with different frequency of porcine nephropathy in Bulgaria

Haematological, biochemical and toxicological investigations of blood and urine of normally slaughtered pigs exhibiting different frequency (1-2%, 10-20% and 50-60%) of changes characterized as "enlarged mottled kidneys"; at the slaughtering meat inspection were carried out to elucidate the nature of nephropathies encountered in Bulgaria. A content of ochratoxin A, higher in the spring than the autumn, was found in the serum and urine samples. The mean contamination levels of ochratoxin A in consumed feeds ranged from 114 +/- 36 ppb for 1994 to 207 +/- 65 ppb for 1993. The renal changes were characterized by impairment of proximal tubular function (indicated by an increased urinary excretion of glucose and protein) as well as by decreased specific gravity and increased pH in the urine mainly in pigs with 50-60% frequency of nephropathy. The concentration of urea, creatinine and glucose in the blood was increased, whereas the serum protein and cholesterol were decreased in pigs with 10-20% and 50-60% frequency of nephropathy. The mean enzyme levels of gamma-glutamyl transpeptidase and leucine aminopeptidase were significantly increased in the urine. The presence of granular casts and necrotic renal tubular cells were established in the sediment.