Effect of hypoxia on stromal precursors from rat bone marrow at the early stage of culturing

被引：17

作者：

Buravkova, L. B. ^{[1
]}

Anokhina, E. B. ^{[1
]}

机构：

[1] Russian Acad Med Sci, State Res Ctr Russian Federat, Inst Med & Biol Problems, Moscow, Russia

来源：

BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE | 2007年 / 143卷 / 04期

关键词：

stromal precursors; mesenchymal stem cells; hypoxia;

D O I：

10.1007/s10517-007-0143-6

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 [基础医学];

摘要：

We studied the effect of hypoxia on stromal precursors from rat bone marrow. Under hypoxic conditions the cultures were characterized by low heterogeneity, the percent of large spread morphologically different cells decreased. Hypoxia increased proliferative activity of cells, had no adverse effect, and decreased the percent of cells expressing hemopoietic markers.

引用

页码：411 / 413

页数：3

共 13 条

[1]

Hypoxia promotes murine bone-marrow-derived stromal cell migration and tube formation [J].

Annabi, B ;

Lee, YT ;

Turcotte, S ;

Naud, E ;

Desrosiers, RR ;

Champagne, M ;

Eliopoulos, N ;

Galipeau, J ;

Béliveau, R .

STEM CELLS, 2003, 21 (03) :337-347

[2]

Mesenchymal stem cells: clinical applications and biological characterization [J].

Barry, FP ;

Murphy, JM .

INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, 2004, 36 (04) :568-584

[3]

Bone marrow stromal stem cells: Nature, biology, and potential applications [J].

Bianco, P ;

Riminucci, M ;

Gronthos, S ;

Robey, PG .

STEM CELLS, 2001, 19 (03) :180-192

[4]

CHERTKOV IL, 2005, ROS AKAD MED NAUK, P37

[5]

The expansion of murine bone marrow cells preincubated in hypoxia as an in vitro indicator of their marrow-repopulating ability [J].

Cipolleschi, MG ;

Rovida, E ;

Ivanovic, Z ;

Praloran, V ;

Olivotto, M ;

Dello Sbarba, P .

LEUKEMIA, 2000, 14 (04) :735-739

[6]

The role of hypoxia inducible factor 1 (HIF-1) in hypoxia induced apoptosis [J].

Greijer, AE ;

van der Wall, E .

JOURNAL OF CLINICAL PATHOLOGY, 2004, 57 (10) :1009-1014

[7]

Rat marrow stromal cells are more sensitive to plating density and expand more rapidly from single-cell-derived colonies than human marrow stromal cells [J].

Javazon, EH ;

Colter, DC ;

Schwarz, EJ ;

Prockop, DJ .

STEM CELLS, 2001, 19 (03) :219-225

[8]

LAGARKOVA MA, 2006, KLET TEKHNOL BIOL ME, P3

[9]

Cultivation of rat marrow-derived mesenchymal stem cells in reduced oxygen tension: Effects on in vitro and in vivo osteochondrogenesis [J].

Lennon, DP ;

Edmison, JM ;

Caplan, AI .

JOURNAL OF CELLULAR PHYSIOLOGY, 2001, 187 (03) :345-355

[10]

Mesenchymal stem cells [J].

Minguell, JJ ;

Erices, A ;

Conget, P .

EXPERIMENTAL BIOLOGY AND MEDICINE, 2001, 226 (06) :507-520

← 1 2 →