Use of a semi automated, robotic radioimmunoassay to measure cAMP generated by activation of DP-, EP2-, and IP-prostaglandin receptors in human ocular and other cell types

被引：17

作者：

Crider, JY ^{[1
]}

Griffin, BW ^{[1
]}

Xu, SX ^{[1
]}

Sharif, NA ^{[1
]}

机构：

[1] Alcon Labs Inc, Ft Worth, TX 76134 USA

来源：

PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS | 1998年 / 59卷 / 01期

关键词：

D O I：

10.1016/S0952-3278(98)90055-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The aim of these studies was to compare the effects of several prostaglandin agonists on adenylyl cyclase activity in embryonic bovine tracheal (EBTr) cells, transformed human nonpigmented ciliary epithelial (NPE) cells and National Cancer Bank (NCB-20) cells. These cell types have been shown to express DP, EP, and IP prostaglandin (PG) receptors, respectively. Cyclic AMP (cAMP) generation was measured by manual and semiautomated radioimmunoassay (RIA) techniques. ZK118182 (EC50 = 10-27 nM), PGE(2) (EC50 = 21-27 nM) and PGI(2) (EC50 = 3.5-4 nM) had the highest potency at the DP, EP2 and IP receptors, respectively. A plot of potency (EC50) values generated with both techniques showed a high degree of correlation for all three receptors. These studies provide further characterization of prostanoid receptor functional responses in three cell types and demonstrate the advantages of a semi-automated RIA method for the analysis of the second messenger cAMP.

引用

页码：77 / 82

页数：6

共 18 条

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