Simple gas chromatography method for lipase assay

被引：9

作者：

Kulkarni, N ^{[1
]}

Gadre, RV ^{[1
]}

机构：

[1] Natl Chem Lab, Poona 411008, Maharashtra, India

来源：

BIOTECHNOLOGY TECHNIQUES | 1998年 / 12卷 / 08期

关键词：

D O I：

10.1023/A:1008884130425

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A gas chromatography method for lipase assay using tributyrin as substrate is presented. Tributyrin is hydrolyzed by lipase to produce free butyric acid that is directly quantified by gas chromatography. The estimation of lipase activity takes only 6 min after enzyme reaction. The technique needs a small enzyme sample and is useful for analysis of large number of lipase samples.

引用

页码：627 / 628

页数：2

共 5 条

[1] BACTERIAL LIPASES [J].