Efficient Yeast One-/Two-Hybrid Screening Using a Library Composed Only of Transcription Factors in Arabidopsis thaliana

被引：95

作者：

Mitsuda, Nobutaka ^{[1
]}

Ikeda, Miho ^{[1
]}

Takada, Shinobu ^{[3
]}

Takiguchi, Yuko ^{[1
]}

Kondou, Youichi ^{[2
]}

Yoshizumi, Takeshi ^{[2
]}

Fujita, Miki ^{[2
]}

Shinozaki, Kazuo ^{[2
]}

Matsui, Minami ^{[2
]}

Ohme-Takagi, Masaru ^{[1
]}

机构：

[1] Natl Inst Adv Ind Sci & Technol, Bioprod Res Inst, Tsukuba, Ibaraki 3058562, Japan

[2] RIKEN, Yokohama Inst, Plant Sci Ctr, Tsurumi Ku, Kanagawa 2300045, Japan

[3] Osaka Univ, Grad Sch Sci, Osaka, Japan

来源：

PLANT AND CELL PHYSIOLOGY | 2010年 / 51卷 / 12期

基金：

日本学术振兴会;

关键词：

Arabidopsis; Methodology; Transcription factor; Yeast one-hybrid screening; FUNCTIONAL-ANALYSIS; GENE; EXPRESSION; ACTIVATION; REPRESSORS; LIGNIN; SYSTEM; DOMAIN;

D O I：

10.1093/pcp/pcq161

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Yeast one-hybrid screening is widely used for the identification of transcription factors (TFs) that interact with specific DNA sequences. However, screening a whole cDNA library is not efficient for the identification of TFs because TF genes represent only a small percentage of clones in a cDNA library. Here, we present the development of an efficient yeast one-hybrid screening system using a prey library composed only of approximately 1,500 TF cDNAs of Arabidopsis thaliana. This library enabled us to isolate a TF that binds to a specific promoter sequence with high efficiency, even when the promoter region of the gene of interest was directly employed as bait. Furthermore, this library was also successfully applied as a yeast two-hybrid library to find TFs that interact with specific proteins. This efficient system will contribute to the elucidation of gene regulatory networks in plants.

引用

页码：2145 / 2151

页数：7

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