Functional evidence for indirect recognition of G center dot U in tRNA(Ala) by alanyl-tRNA synthetase

被引：103

作者：

Gabriel, K ^{[1
]}

Schneider, J ^{[1
]}

McClain, WH ^{[1
]}

机构：

[1] UNIV WISCONSIN, DEPT BACTERIOL, MADISON, WI 53706 USA

来源：

SCIENCE | 1996年 / 271卷 / 5246期

关键词：

D O I：

10.1126/science.271.5246.195

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The structural features of the G . U wobble pair in Escherichia coli alanine transfer RNA (tRNA(Ala)) that are associated with aminoacylation by alanyl-tRNA synthetase (AlaRS) were investigated in vivo for wild-type tRNA(Ala) and mutant tRNAs with G . U substitutions. tRNA(Ala) with G . U, C . A, or G . A gave similar amounts of charged tRNA(Ala) and supported viability of E. coli lacking chromosomal tRNA(Ala) genes. tRNA(Ala) With G . C was inactive. Recognition of G . U by AlaRS thus requires more than the functional groups on G . U in a regular helix and may involve detection of a helical distortion.

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页码：195 / 197

页数：3

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