Enrichment of gene-coding sequences in maize by genome filtration

被引:156
作者
Whitelaw, CA
Barbazuk, WB
Pertea, G
Chan, AP
Cheung, F
Lee, Y
Zheng, L
van Heeringen, S
Karamycheva, S
Bennetzen, JL
SanMiguel, P
Lakey, N
Bedell, J
Yuan, Y
Budiman, MA
Resnick, A
Van Aken, S
Utterback, T
Riedmuller, S
Williams, M
Feldblyum, T
Schubert, K
Beachy, R
Fraser, CM
Quackenbush, J
机构
[1] Inst Genom Res TIGR, Rockville, MD 20850 USA
[2] Donald Danforth Plant Sci Ctr, St Louis, MO 63132 USA
[3] Univ Georgia, Dept Genet, Athens, GA 30602 USA
[4] Purdue Univ, Purdue Genom Core Facil, W Lafayette, IN 47907 USA
[5] Orion Genom, St Louis, MO 63108 USA
[6] J Craig Venter Sci fdn Joint Technol Ctr, Rockville, MD 20850 USA
关键词
D O I
10.1126/science.1090047
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Approximately 80% of the maize genome comprises highly repetitive sequences interspersed with single-copy, gene-rich sequences, and standard genome sequencing strategies are not readily adaptable to this type of genome. Methodologies that enrich for genic sequences might more rapidly generate useful results from complex genomes. Equivalent numbers of clones from maize selected by techniques called methylation filtering and High C(0)t selection were sequenced to generate similar to 200,000 reads ( approximately 132 megabases), which were assembled into contigs. Combination of the two techniques resulted in a sixfold reduction in the effective genome size and a fourfold increase in the gene identification rate in comparison to a nonenriched library.
引用
收藏
页码:2118 / 2120
页数:3
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