Arabidopsis RAD51C gene is important for homologous recombination in meiosis and mitosis

被引:81
作者
Abe, K
Osakabe, K
Nakayama, S
Endo, M
Tagiri, A
Todoriki, S
Ichikawa, H
Toki, S [1 ]
机构
[1] Natl Inst Agrobiol Sci, Dept Plant Biotechnol, Tsukuba, Ibaraki 3058602, Japan
[2] Natl Inst Agrobiol Sci, Genet Divers Dept, Tsukuba, Ibaraki 3058602, Japan
[3] Univ Tsukuba, Grad Sch Life Environm Sci, Tsukuba, Ibaraki 3050005, Japan
[4] Natl Food Res Inst, Food Hyg Team, Tsukuba, Ibaraki 3058642, Japan
关键词
D O I
10.1104/pp.105.065243
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Rad51 is a homolog of the bacterial RecA recombinase, and a key factor in homologous recombination in eukaryotes. Rad51 paralogs have been identified from yeast to vertebrates. Rad51 paralogs are thought to play an important role in the assembly or stabilization of Rad51 that promotes homologous pairing and strand exchange reactions. We previously characterized two RAD51 paralogous genes in Arabidopsis (Arabidopsis thaliana) named AtRAD51C and AtXRCC3, which are homologs of human RAD51C and XRCC3, respectively, and described the interaction of their products in a yeast two-hybrid system. Recent studies showed the involvement of AtXrcc3 in DNA repair and functional role in meiosis. To determine the role of RAD51C in meiotic and mitotic recombination in higher plants, we characterized a T-DNA insertion mutant of AtRAD51C. Although the atrad51C mutant grew normally during vegetative developmental stage, the mutant produced aborted siliques, and their anthers did not contain mature pollen grains. Crossing of the mutant with wild-type plants showed defective male and female gametogeneses as evidenced by lack of seed production. Furthermore, meiosis was severely disturbed in the mutant. The atrad51C mutant also showed increased sensitivity to gamma-irradiation and cisplatin, which are known to induce double-strand DNA breaks. The efficiency of homologous recombination in somatic cells in the mutant was markedly reduced relative to that in wild-type plants.
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页码:896 / 908
页数:13
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