In vivo cardiovasculogenesis by direct injection of isolated adult mesenchymal stem cells

被引：223

作者：

Gojo, S

Gojo, N

Takeda, Y

Mori, T

Abe, H

Kyo, S

Hata, J

Umezawa, A

机构：

[1] Keio Univ, Sch Med, Dept Pathol, Shinjuku Ku, Tokyo 1608582, Japan

[2] Natl Res Inst Child Hlth & Dev, Tokyo, Japan

[3] Saitama Med Ctr, Dept Cardiovasc Surg, Moroyama, Saitama, Japan

来源：

EXPERIMENTAL CELL RESEARCH | 2003年 / 288卷 / 01期

关键词：

regeneration; transplantation; cell therapy; cardiomyocyte; marrow stroma;

D O I：

10.1016/S0014-4827(03)00132-0

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The characterization of mesenchymal stem cells (MSCs) is of biological and clinical interest. We demonstrate that isolated MSCs, defined by CD34(low) c-kit(+) CD140a(+) Sca-1(high), are able to differentiate into cardiomyocytes, endothelial cells, and pericytes or smooth muscle cells by direct injection into adult heart. In skeletal muscle and lung, they also contributed to formation of the vasculature. MSCs did not transform into malignant cells or form excess extracellular matrix. This study suggests that MSCs may supply an ideal donor source of cardiovascular cells in patients with cardiopulmonary diseases. (C) 2003 Elsevier Science (USA). All rights reserved.

引用

页码：51 / 59

页数：9

共 36 条

[1] PATTERNS OF DNA METHYLATION ARE INDISTINGUISHABLE IN DIFFERENT INDIVIDUALS OVER A WIDE-RANGE OF HUMAN DNA-SEQUENCES [J].

BEHNKRAPPA, A ;

HOLKER, I ;

DESILVA, US ;

DOERFLER, W .

GENOMICS, 1991, 11 (01) :1-7

[2] Recruitment of bone-marrow-derived cells by skeletal and cardiac muscle in adult dystrophic mdx mice [J].

Bittner, RE ;

Schöfer, C ;

Weipoltshammer, K ;

Ivanova, S ;

Streubel, B ;

Hauser, E ;

Freilinger, M ;

Höger, H ;

Elbe-Bürger, A ;

Wachtler, F .

ANATOMY AND EMBRYOLOGY, 1999, 199 (05) :391-396

[3] Generalized potential of adult neural stem cells [J].

Clarke, DL ;

Johansson, CB ;

Wilbertz, J ;

Veress, B ;

Nilsson, E ;

Karlström, H ;

Lendahl, U ;

Frisén, J .

SCIENCE, 2000, 288 (5471) :1660-1663

[4]

Cohnheim J, 1867, VIRCHOWS ARCH, V40, P1

[5] DNA METHYLATION CONTROLS THE INDUCIBILITY OF THE MOUSE METALLOTHIONEIN-I GENE IN LYMPHOID-CELLS [J].

COMPERE, SJ ;

PALMITER, RD .

CELL, 1981, 25 (01) :233-240

[6] Bone marrow stromal cells as a vehicle for gene transfer [J].

Ding, L ;

Lu, S ;

Batchu, RB ;

Saylors, RL ;

Munshi, NC .

GENE THERAPY, 1999, 6 (09) :1611-1616

[7]

FRIEDENSTEIN AJ, 1976, EXP HEMATOL, V4, P267

[8] Transplantation of genetically marked cardiac muscle cells [J].

Gojo, S ;

Kitamura, S ;

Hatano, O ;

Takakusu, A ;

Hashimoto, K ;

Kanegae, Y ;

Saito, I .

JOURNAL OF THORACIC AND CARDIOVASCULAR SURGERY, 1997, 113 (01) :10-18

[9] Bone marrow-derived regenerated cardiomyocytes (CMG cells) express functional adrenergic and muscarinic receptors [J].

Hakuno, D ;

Fukuda, K ;

Makino, S ;

Konishi, F ;

Tomita, Y ;

Manabe, T ;

Suzuki, Y ;

Umezawa, A ;

Ogawa, S .

CIRCULATION, 2002, 105 (03) :380-386

[10] Pluripotency of mesenchymal stem cells derived from adult marrow [J].

Jiang, Yuehua ;

Jahagirdar, Balkrishna N. ;

Reinhardt, R. Lee ;

Schwartz, Robert E. ;

Keene, C. Dirk ;

Ortiz-Gonzalez, Xilma R. ;

Reyes, Morayma ;

Lenvik, Todd ;

Lund, Troy ;

Blackstad, Mark ;

Du, Jingbo ;

Aldrich, Sara ;

Lisberg, Aaron ;

Low, Walter C. ;

Lergaespada, David A. ;

Verfaillie, Catherine M. .

Nature, 2002, 418 (6893) :41-49

← 1 2 3 4 →