Growth phase-dependent changes in the subcellular localization of pre-B-cell colony-enhancing factor

被引：156

作者：

Kitani, T

Okuno, S

Fujisawa, H ^{[1
]}

机构：

[1] Asahikawa Med Coll, Dept Biochem, Asahikawa, Hokkaido 0788510, Japan

[2] Shiga Med Ctr, Res Inst, Moriyama 5248524, Japan

来源：

FEBS LETTERS | 2003年 / 544卷 / 1-3期

关键词：

pre-B-cell colony-enhancing factor; cell cycle; subcellular distribution; cell nucleus;

D O I：

10.1016/S0014-5793(03)00476-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A cDNA encoding the homolog of the human pre-B-cell colony-enhancing factor (PBEF), a cytokine-like secreted protein, was isolated from a rat cDNA library. This protein existed in both the cytoplasm and nucleus of the cells, and the amount was higher in the cytoplasm than in the nucleus of proliferating PC-12 and Swiss 3T3 cells but higher in the nucleus than in the cytoplasm of the PC-12 cells treated with nerve growth factor and the 3T3 cells grown to a confluent state. Thus, the so-called PBEF is not a cytokine-like secreted protein but an intracellular protein associated with the cell cycle. (C) 2003 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：74 / 78

页数：5

共 12 条

[1] ESTABLISHMENT OF A NORADRENERGIC CLONAL LINE OF RAT ADRENAL PHEOCHROMOCYTOMA CELLS WHICH RESPOND TO NERVE GROWTH-FACTOR [J].