Identification of the pdxK gene that encodes pyridoxine (vitamin B-6) kinase in Escherichia coli K-12

被引：82

作者：

Yang, Y ^{[1
]}

Zhao, GS ^{[1
]}

Winkler, ME ^{[1
]}

机构：

[1] UNIV TEXAS, SCH MED, DEPT MICROBIOL & MOLEC GENET, HOUSTON, TX 77030 USA

来源：

FEMS MICROBIOLOGY LETTERS | 1996年 / 141卷 / 01期

关键词：

pyridoxal 5'-phosphate; pyridoxal kinase; pyridoxine kinase; pdxK; pdxB; pfkB kinase superfamily;

D O I：

10.1016/0378-1097(96)00203-0

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We isolated a miniTn10(Cm-r) insertion mutant lacking pyridoxine (PN) kinase and cloned the structural gene, designated pdxK, by complementation. P1 transduction and PCR mapping and DNA sequence analysis showed that pdxK was adjacent to the err sugar transport gene (53.95 min). Growth properties of pdxK::miniTn10 mutants supported the hypotheses that PN kinase, which also phosphorylates pyridoxal (PL) and pyridoxamine (PM) in vitro, functions solely in the B-6-vitamer salvage pathway and that E. coli contains an additional PL kinase. The amino acid sequence of PdxK has signature motifs of the PfkB superfamily of carbohydrate kinases, which includes phosphofructokinases and ribokinases, and suggests that three unidentified ORFs of Salmonella typhimurium, Haemophilus influenzae, and Saccharomyces cerevisiae correspond to PN/PL/PM kinases.

引用

页码：89 / 95

页数：7

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