Protochlamydia naegleriophila as etiologic agent of pneumonia

被引:52
作者
Casson, Nicola [1 ,2 ]
Michel, Rolf [3 ]
Mueller, Karl-Dieter [4 ]
Aubert, John David [1 ,2 ]
Greub, Gilbert [1 ,2 ]
机构
[1] Univ Hosp Ctr, Inst Microbiol, Ctr Res Intracellular Bacteria, CH-1011 Lausanne, Switzerland
[2] Univ Lausanne, CH-1011 Lausanne, Switzerland
[3] Fed Armed Forces Med Serv, Cent Inst, Koblenz, Germany
[4] Univ Essen Gesamthsch, Inst Med Mikrobiol, Essen, Germany
关键词
D O I
10.3201/eid1401.070980
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Using ameba coculture, we grew a Naegleria endosymbiont. Phenotypic, genetic, and phylogenetic analyses supported its affiliation as Protochlamydia naegleriophila sp. nov. We then developed a specific diagnostic PCR for Protochlamydia spp. When applied to bronchoalveolar lavages, results of this PCR were positive for 1 patient with pneumonia. Further studies are needed to assess the role of Protochlamydia spp. in pneumonia.
引用
收藏
页码:168 / 172
页数:5
相关论文
共 15 条
[1]   Serological cross-reactivity between different Chlamydia-like organisms [J].
Casson, Nicola ;
Entenza, Jos M. ;
Greub, Gilbert .
JOURNAL OF CLINICAL MICROBIOLOGY, 2007, 45 (01) :234-236
[2]   Parachlamydia acanthamoebae enters and multiplies within pneumocytes and lung fibroblasts [J].
Casson, Nicola ;
Medico, Noel ;
Bille, Jacques ;
Greub, Gilbert .
MICROBES AND INFECTION, 2006, 8 (05) :1294-1300
[3]   Emended description of the order Chlamydiales, proposal of Parachlamydiaceae fam. nov. and Simkaniaceae fam. nov., each containing one monotypic genus, revised taxonomy of the family Chlamydiaceae, including a new genus and five new species, and standards for the identification of organisms [J].
Everett, KDE ;
Bush, RM ;
Andersen, AA .
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1999, 49 :415-440
[4]   Antigenic classification of Rickettsia felis by using monoclonal and polyclonal antibodies [J].
Fang, R ;
Raoult, D .
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY, 2003, 10 (02) :221-228
[5]  
Greub G, 2002, EMERG INFECT DIS, V8, P625
[6]   Microorganisms resistant to free-living amoebae [J].
Greub, G ;
Raoult, D .
CLINICAL MICROBIOLOGY REVIEWS, 2004, 17 (02) :413-+
[7]   Serological hint suggesting that Parachlamydiaceae are agents of pneumonia in polytraumatized intensive care patients [J].
Greub, G ;
Boyadjiev, I ;
La Scola, B ;
Raoult, D ;
Martin, C .
RICKETTSIOLOGY: PRESENT AND FUTURE DIRECTIONS, 2003, 990 :311-319
[8]   Parachlamydia acanthamoeba enters and multiplies within human macrophages and induces their apoptosis [J].
Greub, G ;
Mege, JL ;
Raoult, D .
INFECTION AND IMMUNITY, 2003, 71 (10) :5979-5985
[9]  
Greub G, 2003, EMERG INFECT DIS, V9, P755
[10]   Crescent bodies of Parachlamydia acanthamoeba and its life cycle within Acanthamoeba polyphaga:: an electron micrograph study [J].
Greub, G ;
Raoult, D .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2002, 68 (06) :3076-3084