Genetic and functional linkage of Kir5.1 and Kir2.1 channel subunits

被引：53

作者：

Derst, C

Karschin, C

Wischmeyer, E

Hirsch, JR

Preisig-Müller, R

Rajan, S

Engel, H

Grzeschik, KH

Daut, J

Karschin, A ^{[1
]}

机构：

[1] Max Planck Inst Biophys Chem, Dept Mol Neurobiol Signal Transduct, D-37070 Gottingen, Germany

[2] Univ Marburg, Inst Normal & Pathol Physiol, D-35033 Marburg, Germany

[3] Univ Marburg, Inst Human Genet, D-35033 Marburg, Germany

[4] Univ Munster, Med Policlin, D-48149 Munster, Germany

来源：

FEBS LETTERS | 2001年 / 491卷 / 03期

关键词：

inwardly rectifying K+ channel; Kir5.1; KCNJ16; Kir2.1; in situ hybridization; chromosomal localization;

D O I：

10.1016/S0014-5793(01)02202-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have identified several cDNAs for the human Kir5.1 subunit of inwardly rectifying K+ channels, Alternative splicing of exon 3 and the usage of two alternative polyadenylation sites contribute to cDNA diversity. The hKir5.1 gene KCNJ16 is assigned to chromosomal region 17q23.1-24.2, and is separated by only 34 kb from the hKir2.1 gene (KCNJ2), In the brain, Kir5.1 mRNA is restricted to the evolutionary older parts of the hindbrain, midbrain and diencephalon and overlaps a with Kir2.1 in the superior/inferior colliculus and the pontine region. In the kidney Kir5.1 and Kir2.1 are colocalized in the proximal tubule, When expressed in Xenopus oocytes, Kir5.1 is efficiently targeted to the cell surface and forms electrically silent channels together with Kir2.1, thus negatively controlling Kir2.1 channel activity in native cells. (C) 2001 Federation of European Biochemical Societies, Published by Elsevier Science B.V. All rights reserved.

引用

页码：305 / 311

页数：7

共 28 条

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