Phase separation and association of globular protein aggregates in the presence of polysaccharides:: 1.: Mixtures of preheated β-lactoglobulin and k-carrageenan at room temperature

被引:31
作者
Croguennoc, P
Durand, D
Nicolai, T [1 ]
Clark, A
机构
[1] Univ Maine, CNRS, UMR, F-72085 Le Mans 9, France
[2] Unilever Res Colworth, Sharnbrook MK44 1LQ, Beds, England
关键词
D O I
10.1021/la001674q
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Mixtures of globular protein (beta -lactoglobulin) aggregates and polysaccharides (kappa -carrageenan) were studied using dynamic and static light scattering, size exclusion chromatography, and optical microscopy. Above a critical K-carrageenan concentration the protein aggregates phase separate and form spherical microdomains, which slowly sediment. The effect of concentration and size of the protein aggregates was investigated. The phase separation is initially reversible upon dilution, but the microdomains become progressively more stable until after several days they can no longer be redissolved.
引用
收藏
页码:4372 / 4379
页数:8
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