A truncated HCV core protein elicits a potent immune response with a strong participation of cellular immunity components in mice

被引：24

作者：

Alvarez-Obregón, JC

Dueñas-Carrera, S

Valenzuela, C

Grillo, JM

机构：

[1] Ctr Ingn Genet & Biotecnol, HCV Dept, Vaccine Div, Havana 10600, Cuba

[2] Ctr Ingn Genet & Biotecnol, Clin Trials Div, Havana 10600, Cuba

来源：

VACCINE | 2001年 / 19卷 / 28-29期

关键词：

cytokine gene expression; HCV core protein; immune response;

D O I：

10.1016/S0264-410X(01)00141-4

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The immunogenicity of a truncated HCV core protein (Co.120) was studied in BALB/c and C57BL/6 mice, given three intramuscular injections of antigen, adjuvanted with either aluminum hydroxide or Freund's adjuvant. A rapid antibody response was noted after the first dose, with both strains of mice eventually exhibiting comparable levels of anti-core IgG (titers > 1:100000), with a mixed IgG1/IgG2a subclass response. Spleen cells from Co.120-immunized mice gave a significant specific proliferative response. IFN-gamma gene expression was also detected after an ex-vivo specific stimulation of spleen cells in all immunized mice. This response was independent of dose, H-2 genetic background or type of adjuvant. The results indicated that immunization with the Co.120 protein elicits a potent anti-HCV humoral and cellular immune response. (C) 2001 Elsevier Science Ltd. All rights reserved.

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收藏

页码：3940 / 3946

页数：7

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