Quantitative analysis of infrared absorption coefficient of spider silk fibers

被引：7

作者：

Ene, Roxana ^{[2
]}

Papadopoulos, Periklis ^{[1
]}

Kremer, Friedrich ^{[2
]}

机构：

[1] Max Planck Inst Polymer Res, D-55128 Mainz, Germany

[2] Univ Leipzig, Inst Exp Phys 1, D-04103 Leipzig, Germany

来源：

VIBRATIONAL SPECTROSCOPY | 2011年 / 57卷 / 02期

关键词：

Absorption coefficient; Protein; Conformation; Crystallinity; DRAGLINE SILK; SOLID-STATE; BETA-SHEET; MECHANICAL-PROPERTIES; STRUCTURAL ORGANIZATION; VIBRATIONAL ANALYSIS; ORIENTATION; PROTEINS; CONFORMATION; RAMAN;

D O I：

10.1016/j.vibspec.2011.07.005

中图分类号：

O65 [分析化学];

学科分类号：

070302 [分析化学];

摘要：

Spider silk has a high degree of crystallinity comprising nanocrystals which are interlinked by amorphous regions containing pre-strained spidroin chains. Both, crystal volume fraction and crystal size were shown to be critical for the macroscopic mechanical properties of silk. In this study, for the first time, quantitative results were obtained from absorption IR spectra. Two main difficulties must be overcome: the cylindrical sample geometry and the absence of suitable reference spectra. We show that it is possible to get reproducible absorption coefficient spectra from dense parallel fiber grids immersed in a refractive index matching liquid. Comparison with model polypeptides yields the nanocrystal volume fraction that is close to the crystallinity values given by solid-state NMR or X-ray scattering. (C) 2011 Elsevier B.V. All rights reserved,

引用

页码：207 / 212

页数：6

共 54 条

[1]

AMINO ACID COMPOSITION OF SPIDER SILDS [J].

ANDERSEN, SO .

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY, 1970, 35 (03) :705-&

[2]

STRUCTURE OF BETA-POLY-L-ALANINE - REFINED ATOMIC CO-ORDINATES FOR AN ANTI-PARALLEL BETA-PLEATED SHEET [J].

ARNOTT, S ;

DOVER, SD ;

ELLIOTT, A .

JOURNAL OF MOLECULAR BIOLOGY, 1967, 30 (01) :201-&

[3]

Quasistatic and continuous dynamic characterization of the mechanical properties of silk from the cobweb of the black widow spider Latrodectus hesperus [J].

Blackledge, TA ;

Swindeman, JE ;

Hayashi, CY .

JOURNAL OF EXPERIMENTAL BIOLOGY, 2005, 208 (10) :1937-1949

[4]

Orientational order of Australian spider silks as determined by solid-state NMR [J].

Bonev, B ;

Grieve, S ;

Herberstein, ME ;

Kishore, AI ;

Watts, A ;

Separovic, F .

BIOPOLYMERS, 2006, 82 (02) :134-143

[5]

Attenuated total reflection infrared spectroscopy:: An efficient technique to quantitatively determine the orientation and conformation of proteins in single silk fibers [J].

Boulet-Audet, Maxime ;

Lefevre, Thierry ;

Buffeteau, Thierry ;

Pezolet, Michel .

APPLIED SPECTROSCOPY, 2008, 62 (09) :956-962

[6]

Correlation of the β-sheet crystal size in silk fibers with the protein amino acid sequence [J].

Drummy, Lawrence F. ;

Farmer, B. L. ;

Naik, Rajesh R. .

SOFT MATTER, 2007, 3 (07) :877-882

[7]

Design of superior spider silk: From nanostructure to mechanical properties [J].

Du, Ning ;

Liu, Xiang Yang ;

Narayanan, Janaky ;

Li, Lian ;

Lim, Matthew Lek Min ;

Li, Daiqin .

BIOPHYSICAL JOURNAL, 2006, 91 (12) :4528-4535

[8]

Structural Origin of the Strain-Hardening of Spider Silk [J].

Du, Ning ;

Yang, Zhen ;

Liu, Xiang Yang ;

Li, Yang ;

Xu, Hong Yao .

ADVANCED FUNCTIONAL MATERIALS, 2011, 21 (04) :772-778

[9]

VIBRATIONAL ANALYSIS OF PEPTIDES, POLYPEPTIDES, AND PROTEINS .11. BETA-POLY(L-ALANINE) AND ITS N-DEUTERATED DERIVATIVE [J].

DWIVEDI, AM ;

KRIMM, S .

MACROMOLECULES, 1982, 15 (01) :186-193

[10]

VIBRATIONAL ANALYSIS OF PEPTIDES, POLYPEPTIDES, AND PROTEINS .10. POLY(GLYCINE-I) AND ITS ISOTOPIC DERIVATIVES [J].

DWIVEDI, AM ;

KRIMM, S .

MACROMOLECULES, 1982, 15 (01) :177-185

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