Two primer pairs to detect OTA producers by PCR method

被引：56

作者：

Dao, HP ^{[1
]}

Mathieu, F ^{[1
]}

Lebrihi, A ^{[1
]}

机构：

[1] Ecole Natl Super Agron Toulouse, Inst Natl Polytech Toulouse, Equipe Genie Syst Microbiens, Lab Genie Chim,UMR5503,CNRS,INPT,UPS, F-31326 Castanet Tolosan, France

来源：

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY | 2005年 / 104卷 / 01期

关键词：

ochratoxin A; citrinin; Aspergillus ochraceus; PCR; detection;

D O I：

10.1016/j.ijfoodmicro.2005.02.004

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Fungi contaminating foods and feeds may produce many mycotoxins including ochratoxin A (OTA). Early and rapid detection of potential OTA producing fungi is important to reduce the negative impacts of OTA. In this study, two PCR specific primer pairs, AoLC35-12L/AoLC35-12R and AoOTAL/AoOTAR, were designed from a DNA sequence of a polyketide synthase gene in Aspergillus ochraceus NRRL 3174. On 14 different fungi tested by PCR, AoLC35-12L/AoLC35-12R amplified a unique band from either OTA or citrinin producers while AoOTAL/AoOTAR amplified one PCR product only from A. ochraceus. So these primers could be used to detect both OTA and citrinin producing fungi (AoLC35-12L/AoLC35-12R) or only A. ochraceus (AoOTAL/AoOTAR) from foodstuffs using PCR method. (c) 2005 Elsevier B.V. All rights reserved.

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收藏

页码：61 / 67

页数：7

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