A previously found thylakoid membrane protein of 14 kDa (TMP14) is a novel subunit of plant photosystem I and is designated PSI-P

被引:50
作者
Khrouchtchova, A
Hansson, M
Paakkarinen, V
Vainonen, JP
Zhang, SP
Jensen, PE
Scheller, HV
Vener, AV
Aro, EM
Haldrup, A
机构
[1] Royal Vet & Agr Univ, Plant Biochem Lab, Dept Plant Biol, DK-1871 Frederiksberg, Denmark
[2] Linkoping Univ, Fac Hlth Sci, Div Cell Biol, SE-58185 Linkoping, Sweden
[3] Turku Univ, Dept Biol Plant Physiol & Mol Biol, FI-20014 Turku, Finland
来源
FEBS LETTERS | 2005年 / 579卷 / 21期
基金
芬兰科学院; 瑞典研究理事会;
关键词
photosystem I; protein phosphorylation; state transitions; thylakoids; Arabidopsis;
D O I
10.1016/j.febslet.2005.07.061
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We show that the thylakoid membrane phosphoprotein TMP14 is a novel subunit of plant photosystem I (PSI). Blue native/SDS-PAGE and sucrose gradient fractionation demonstrated the association of the protein exclusively with PSI. We designate the protein PSI-P. The presence of PSI-P subunit in Arabidopsis mutants lacking other PSI subunits was analyzed and suggested a location in the proximity of PSI-L, -H and -O subunits. The PSI-P protein was not differentially phosphorylated in state 1 and state 2. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码:4808 / 4812
页数:5
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