Quantification of the detrimental effect of a single primer-template mismatch by real-time PCR using the 16S rRNA gene as an example

被引：209

作者：

Bru, D. ^{[1
]}

Martin-Laurent, F. ^{[1
]}

Philippot, L. ^{[1
]}

机构：

[1] Univ Burgundy, INRA, CMSE, F-21065 Dijon, France

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2008年 / 74卷 / 05期

关键词：

D O I：

10.1128/AEM.02403-07

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We investigated the effects of internal primer-template mismatches on the efficiency of PCR amplification using the 16S rRNA gene as the model template DNA. We observed that the presence of a single mismatch in the second half of the primer extension sequence can result in an underestimation of up to 1,000-fold of the gene copy number, depending on the primer and position of the mismatch.

引用

页码：1660 / 1663

页数：4

共 16 条

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