New trends in affinity sensing: aptamers for ligand binding

被引：196

作者：

Luzi, E ^{[1
]}

Minunni, M ^{[1
]}

Tombelli, S ^{[1
]}

Mascini, M ^{[1
]}

机构：

[1] Univ Florence, Dipartimento Chim, Lab Biosensori, I-30019 Florence, Italy

来源：

TRAC-TRENDS IN ANALYTICAL CHEMISTRY | 2003年 / 22卷 / 11期

关键词：

aptamers; aptasensors; aptazymes; biosensors; in vitro selection; SELEX;

D O I：

10.1016/S0165-9936(03)01208-1

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Aptamers are artificial nucleic acid ligands that can be generated against amino acids, drugs, proteins and other molecules. They are isolated from complex libraries of synthetic nucleic acids by an iterative process of adsorption, recovery and amplification. This review described the in vitro process to obtain aptamers (SELEX). It mentions the main characteristics of these molecules (i.e. affinity, specificity and stability). Moreover, it discusses advantages over antibodies. It reports potential applications of aptamers in analytical and diagnostic assays as biocomponents of biosensors (aptasensors) and allosteric ribozymes (aptazymes). (C) 2003 Published by Elsevier B.V.

引用

页码：810 / 818

页数：9

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