Shared-epitope HLA-DRB1 alleles and sex ratio in Italian patients with rheumatoid arthritis

被引:16
作者
Bongi, SM
Porfirio, B [1 ]
Rombolà, G
Palasciano, A
Beneforti, E
Bianucci, G
机构
[1] Univ Florence, Dept Clin Physiopathol, Human Genet Unit, I-50139 Florence, Italy
[2] Univ Florence, Dept Clin Physiopathol, Human Genet Unit, I-50139 Florence, Italy
[3] Careggi Hosp, Immunogenet Unit, Florence, Italy
关键词
rheumatoid arthritis; HLA-DRB1; shared-epitope; immunogenetics;
D O I
10.1016/S1297-319X(03)00099-X
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Objective. - To investigate the association between the HLA-DRB I alleles sharing the epitope (Q/R)(K/R)RAA and rheumatoid arthritis (RA) in a large sample of Italian patients (N = 264) recruited from a single centre over the last 5 years. Methods. - Patients' classification according to the ACR criteria. DNA typing of HLA-DRB 1 alleles by conventional polymerase chain reaction sequence specific oligonucleotide probing techniques. Results. - Low-resolution DRB 1 "generic" typing showed a significantly higher frequency of DR4+ RA patients as compared to normal controls. Both DRI and DR 10 specificities were over-represented in our patients, but neither reached the statistically significant P level of 0.05 after Bonferroni's correction. However, direct search of Q(K/R)RAA epitopes, which are present in most DR4+ and DRI+ samples, demonstrated that these motifs were found at increased frequencies in RA patients. Stratification according to gender did not show differences in the proportion of disease-associated HLA alleles. Conclusions. - Our study confirms the association of HLA-DR4, and -DRI alleles, and more generally speaking of the shared epitopes Q(K/R)RAA, with disease susceptibility in Italian patients. (C) 2003 Elsevier SAS. All rights reserved.
引用
收藏
页码:24 / 28
页数:5
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