[1] Tsing Hua Univ, Dept Biol Sci & Biotechnol, Lab Pharmaceut Sci, Beijing 100084, Peoples R China
[2] Chinese Acad Med Sci, Peking Union Med Coll, Inst Med Plant, Beijing 100094, Peoples R China
来源:
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
|
2003年
/
796卷
/
01期
关键词:
ellagic acid;
pharmacokinetics;
D O I:
10.1016/S1570-0232(03)00610-X
中图分类号:
Q5 [生物化学];
学科分类号:
071010 ;
081704 ;
摘要:
Quantification of ellagic acid, the principal bioactive component of pomegranate leaf extract, in rats plasma following oral administration of pomegranate leaf extract was achieved by using a high-performance liquid chromatographic method. The calibration curve for ellagic acid was linear (r(2) = 0.9998) ver the concentration range 0.026-1.3 mug/ml. The intra- and inter-day assays of ellagic acid from rat plasma were less than 6.52% at concentration range from 26 to 1300 ng/ml and good overall recoveries (94.5-102.4%) were found on same concentrations. The concentration-time profile was fitted with an open two-compartment system with lag time and its max concentration of ellagic acid in plasma was 213 ng/ml only 0.55 h after oral administration extract 0.8 g/kg. The pharmacokinetic profile indicates that ellagic acid has poor absorption and rapid elimination after oral administration pomegranate leaf extract, and part of it was absorbed from stomach. (C) 2003 Elsevier B.V. All rights reserved.