Processing of serum proteins underlies the mass spectral fingerprinting of myocardial infarction

被引：167

作者：

Marshall, J ^{[1
]}

Kupchak, P ^{[1
]}

Zhu, WM ^{[1
]}

Yantha, J ^{[1
]}

Vrees, T ^{[1
]}

Furesz, S ^{[1
]}

Jacks, K ^{[1
]}

Smith, C ^{[1
]}

Kireeva, I ^{[1
]}

Zhang, R ^{[1
]}

Takahashi, M ^{[1
]}

Stanton, E ^{[1
]}

Jackowski, G ^{[1
]}

机构：

[1] SYNX PHARMA, Toronto, ON M9W 1E7, Canada

来源：

JOURNAL OF PROTEOME RESEARCH | 2003年 / 2卷 / 04期

关键词：

mass spectrometry; myocardial infarction; diagnostic; fibrinogen alpha peptide; complement C3f; sera;

D O I：

10.1021/pr030003l

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The MALDI-TOF spectra of peptides from the sera of normal and myocardial infarction patients produced patterns that provided an accurate diagnostic of MI. In myocardial infarction, the spectral pattern originated from the cleavage of complement C3 alpha chain to release the C3f peptide and cleavage of fibrinogen to release peptide A. The fibrinogen peptide A and complement C3f peptide were in turn progressively truncated by aminopeptidases to produce two families of fragments that formed the characteristic spectral pattern of MI. Time course and inhibitor studies demonstrated that the peptide patterns in the serum reflect the balance of disease-specific-protease and aminopeptidase activity ex vivo.

引用

页码：361 / 372

页数：12

共 56 条

[1]

[Anonymous], MULTIVARIATE ANAL 2

[2]

Ardekani Ali M, 2002, Expert Rev Mol Diagn, V2, P312, DOI 10.1586/14737159.2.4.312

[3] Cardiac markers of acute coronary syndromes: is there a case for point-of-care testing? [J].