Towards a mutant analysis of the tertiary structures of functional DNA-binding motifs

被引：2

作者：

Barker, A ^{[1
]}

Müller-Hill, B ^{[1
]}

机构：

[1] Univ Koln, Genet Inst, D-50931 Cologne, Germany

来源：

FEBS LETTERS | 1998年 / 432卷 / 1-2期

关键词：

transcription factor; recognition helix; induced fit; unstructured protein region; cold sensitive mutant; heat sensitive mutant;

D O I：

10.1016/S0014-5793(98)00797-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Transcription factors have specific regions, often alpha helices? with which they recognise DNA, These regions are more or less disordered off DNA. Some examples are listed here, However, a detailed mutant analysis of this phenomenon is missing. It could show to what extent DNA binding in vitro and in vivo is harmed when such a region is artificially made rigid by suitable substitutions and could reveal how much transcription factors have improved by having been selected to carry unstable recognition domains. (C) 1998 Federation of European Biochemical Societies.

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页码：1 / 3

页数：3

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