High efficient expression of cellobiase gene from Aspergillus niger in the cells of Trichoderma reesei

被引：103

作者：

Wang, Bingbing ^{[1
]}

Xia, Liming ^{[1
]}

机构：

[1] Zhejiang Univ, Dept Chem Engn & Bioengn, Hangzhou 310027, Zhejiang, Peoples R China

来源：

BIORESOURCE TECHNOLOGY | 2011年 / 102卷 / 06期

基金：

国家高技术研究发展计划(863计划);

关键词：

Cellobiase; Aspergillus niger; Transformation; Trichoderma reesei; Gene expression; EXTRACELLULAR BETA-GLUCOSIDASE; ENZYMATIC-HYDROLYSIS; CELLULASE PRODUCTION; CLONING; SACCHARIFICATION;

D O I：

10.1016/j.biortech.2010.12.099

中图分类号：

S2 [农业工程];

学科分类号：

082806 [农业信息与电气工程];

摘要：

The cellobiase gene from Aspergillus niger was cloned and connected with the strong promoter Pcbh1 from Trichoderma reesei to construct a recombinant plasmid pHB9 with the hygromycin B resistance marker. The plasmid was transformed into conidia of T. reesei using the modified PEG-CaCl2 method. Main factors effecting the transformation were discussed and about 99-113 transformants/mu g DNA could be obtained under optimal conditions. It was found that the molecular mass of the recombinant cellobiase was about 120 kDa by SDS-PAGE analysis. The activity of cellobiase could reach 5.3 IU/ml after 48 h fermentation, which was as high as 106 times compared with that of the host strain. Meanwhile, the filter paper activity of recombinant T. reesei was 1.44-fold of the host strain. Saccharification of corncob residue with the crude enzyme showed that the hydrolysis yield (84.2%) of recombinant T. reesei was 21% higher than that (69.5%) of the host strain. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：4568 / 4572

页数：5

共 29 条

[1]

CLONING AND AMPLIFICATION OF THE GENE ENCODING AN EXTRACELLULAR BETA-GLUCOSIDASE FROM TRICHODERMA-REESEI - EVIDENCE FOR IMPROVED RATES OF SACCHARIFICATION OF CELLULOSIC SUBSTRATES [J].