Structural features of an arabinan fragment isolated from the water-soluble fraction of dehulled rapeseed

被引:30
作者
Eriksson, I [1 ]
Andersson, R [1 ]
Westerlund, E [1 ]
Andersson, R [1 ]
Aman, P [1 ]
机构
[1] SWEDISH UNIV AGR SCI,DEPT CHEM,S-75007 UPPSALA,SWEDEN
关键词
rapeseed; arabinan; NMR;
D O I
10.1016/0008-6215(95)00337-1
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A water-soluble polysaccharide fraction was prepared from dehulled rapeseed meal (winter rapeseed variety Casino). Further purification yielded two major fractions having a high content of arabinose and galactose residues, with Ara/Gal ratios of 5.4 (G1) and 1.8 (G2), The Ara/Gal ratio of the high molecular weight fraction G1 was stable over the whole gel filtration peak, indicating that the arabinose and galactose residues are part of the same polysaccharide. The high molecular weight fraction G1 was studied further by methylation analysis and several NMR techniques. Structural studies showed G1 to consist mainly of arabinan fragments, which have terminal alpha-L-arabinofuranosyl groups with anomeric carbons bound (1 --> 5) (A) or (1 --> 2) (B), and 2,5-substituted arabinosyl residues with anomeric carbons bound (1 --> 5) (D) or (1 --> 2) (C) to adjacent arabinosyl residues. The A:B:C:D ratios were 2:1:1:1 according to results from NMR and methylation analysis.
引用
收藏
页码:161 / 172
页数:12
相关论文
共 28 条
[1]  
BJERGEGAARD C, 1992, P GCIRC 8 INT RAP C, V2, P448
[2]   POLYSACCHARIDES FROM THE ROOTS OF THE MARSH MALLOW (ALTHAEA-OFFICINALIS L) - STRUCTURE OF AN ARABINAN [J].
CAPEK, P ;
TOMAN, R ;
KARDOSOVA, A ;
ROSIK, J .
CARBOHYDRATE RESEARCH, 1983, 117 (JUN) :133-140
[3]  
CHURMS SC, 1983, CARBOHYD RES, V113, P339, DOI 10.1016/0008-6215(83)88251-2
[4]   FORM AND FUNCTION OF ARABINOGALACTAN-PROTEINS [J].
CLARKE, AE ;
ANDERSON, RL ;
STONE, BA .
PHYTOCHEMISTRY, 1979, 18 (04) :521-540
[5]   CHEMICAL-COMPOSITION IN VARIETIES OF RAPESEED AND TURNIP RAPESEED, INCLUDING SEVERAL SAMPLES OF HULL AND DEHULLED SEED [J].
ERIKSSON, I ;
WESTERLUND, E ;
AMAN, P .
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, 1994, 66 (02) :233-240
[6]   ARABINOGALACTAN-PROTEINS - STRUCTURE, BIOSYNTHESIS, AND FUNCTION [J].
FINCHER, GB ;
STONE, BA ;
CLARKE, AE .
ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 1983, 34 :47-70
[7]  
Gorin P. A. J., 1981, ADV CARBOHYD CHEM BI, V38, P13, DOI [DOI 10.1016/S0065-2318(08)60309-1, 10.1016/S0065-2318(08)60309-1]
[8]   C-13 AND PROTON NUCLEAR MAGNETIC-RESONANCE STUDIES ON METHYL ALDOFURANOSIDES AND THEIR O-ALKYL DERIVATIVES [J].
GORIN, PAJ ;
MAZUREK, M .
CARBOHYDRATE RESEARCH, 1976, 48 (02) :171-186
[9]   AN IMPROVED PROCEDURE FOR THE METHYLATION ANALYSIS OF OLIGOSACCHARIDES AND POLYSACCHARIDES [J].
HARRIS, PJ ;
HENRY, RJ ;
BLAKENEY, AB ;
STONE, BA .
CARBOHYDRATE RESEARCH, 1984, 127 (01) :59-73
[10]  
Jansson P.-E., 1976, CHEM COMMUN, V8, P1