Determination of genetic relationships among Phaseolus vulgaris populations in a conical cross from RAPD marker analyses

被引：15

作者：

Bai, YH ^{[1
]}

Michaels, TE ^{[1
]}

Pauls, KP ^{[1
]}

机构：

[1] Univ Guelph, Dept Crop Sci, Guelph, ON N1G 2W1, Canada

来源：

MOLECULAR BREEDING | 1998年 / 4卷 / 05期

基金：

加拿大自然科学与工程研究理事会;

关键词：

common bean; conical cross; genetic distance; RAPD markers;

D O I：

10.1023/A:1009601910980

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Random-amplified polymorphic DNA (RAPD) markers were used to determine genetic relationships among Phaseolus vulgaris breeding populations. Genetic distances were calculated from the distribution of 317 RAPD markers among 8 parents, 10 individuals from 8 cycle-one populations, 10 individuals from 6 cycle-two populations and 10 individuals from 2 cycle-three populations of a conical cross. Genetic distances between populations and parents were consistent with their degree of relationship in the crossing scheme indicating that a RAPD analysis is a sensitive and useful method for categorizing breeding materials according to their genetic similarities. Genetic variation among individuals within populations increased from cycle one to cycle three and variation among populations within the cycles decreased from cycle one to cycle three in the conical cross. The results showed that this crossing scheme can be used to collect the genetic diversity in eight parents into a single plant breeding population.

引用

页码：395 / 406

页数：12

共 41 条

[1] Identification of RAPD markers linked to common bacterial blight resistance genes in Phaseolus vulgaris L. [J].