D-alanine deprivation of Bacillus subtilis teichoic acids is without effect on cell growth and morphology but affects the autolytic activity

被引:66
作者
Wecke, J
Perego, M
Fischer, W
机构
[1] UNIV ERLANGEN NURNBERG, FAK MED, INST BIOCHEM, D-91054 ERLANGEN, GERMANY
[2] ROBERT KOCH INST, D-13353 BERLIN, GERMANY
[3] UNIV PARMA, DIPARTIMENTO FARMACEUT, I-43100 PARMA, ITALY
关键词
D O I
10.1089/mdr.1996.2.123
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Using insertional inactivation of the different genes of the dlt operon in Bacillus subtilis, we searched for metabolic and morphological changes caused by D-alanine ester deprivation of lipoteichoic acid and wall teichoic acid, There were no alterations of cell growth, basic metabolism, cellular content of phosphorus-containing compounds, ultrastructure, cell separation, and surface charge, The only alteration observed was an enhancement of endogenous and beta-lactam-induced cell lysis, Since this enhancement is doubtless correlated with the D-alanine ester deprivation of the teichoic acids, the present view based on in vitro experiments, that negatively charged LTA is inhibitory to autolysins, may be questioned, We propose that negatively charged lipoteichoic acid and/or wall teichoic acid serve in vivo to fix the cationic autolysins within the cell wail-membrane complex by electrostatic interaction, Positively charged D-alanine ester substituents decrease the binding capacity of the teichoic acids for autolysins by charge compensation.
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页码:123 / 129
页数:7
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