Signaling of cell fate determination by the TPD1 small protein and EMS1 receptor kinase

被引：151

作者：

Jia, Gengxiang ^{[1
]}

Liu, Xiaodong ^{[1
]}

Owen, Heather A. ^{[1
]}

Zhao, Dazhong ^{[1
]}

机构：

[1] Univ Wisconsin, Dept Sci Biol, Milwaukee, WI 53211 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2008年 / 105卷 / 06期

关键词：

Arabidopsis; sexual reproduction; anther; ligand; signal transduction;

D O I：

10.1073/pnas.0708795105

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Sexual reproduction requires the specification of cells with distinct fates in plants and animals. The EMS1 (also known as EXS) leucine-rich repeat receptor-like kinase (LRR-RLK) and TPD1 small protein play key roles in regulating somatic and reproductive cell fate determination in Arabidopsis anthers. Here, we show that ectopic expression of TPD1 causes abnormal differentiation of somatic and reproductive cells in anthers. In addition, ectopic TPD1 activity requires functional EMS1. Yeast two-hybrid, pull-down, and coimmunoprecipitation analyses further demonstrate that TPD1 interacts with EMS1 in vitro and in vivo. Moreover, TPD1 induces EMS1 phosphorylation in planta. Thus, our results suggest that TPD1 serves as a ligand for the EMS1 receptor kinase to signal cell fate determination during plant sexual reproduction.

引用

页码：2220 / 2225

页数：6

共 61 条

[1] The Arabidopsis thaliana SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASES1 and 2 control male sporogenesis [J].

Albrecht, C ;

Russinova, E ;

Hecht, V ;

Baaijens, E ;

de Vries, S .

PLANT CELL, 2005, 17 (12) :3337-3349

[2] Dependence of stem cell fate in Arabidopsis an a feedback loop regulated by CLV3 activity [J].

Brand, U ;

Fletcher, JC ;

Hobe, M ;

Meyerowitz, EM ;

Simon, R .

SCIENCE, 2000, 289 (5479) :617-619

[3] EXS, a putative LRR receptor kinase, regulates male germline cell number and tapetal identity and promotes seed development in Arabidopsis [J].

Canales, C ;

Bhatt, AM ;

Scott, R ;

Dickinson, H .

CURRENT BIOLOGY, 2002, 12 (20) :1718-1727

[4] BRL1 and BRL3 are novel brassinosteroid receptors that function in vascular differentiation in Arabidopsis [J].

Caño-Delgado, A ;

Yin, YH ;

Yu, C ;

Vafeados, D ;

Mora-García, S ;

Cheng, JC ;

Nam, KH ;

Li, JM ;

Chory, J .

DEVELOPMENT, 2004, 131 (21) :5341-5351

[5] The CLAVATA1 gene encodes a putative receptor kinase that controls shoot and floral meristem size in Arabidopsis [J].

Clark, SE ;

Williams, RW ;

Meyerowitz, EM .

CELL, 1997, 89 (04) :575-585

[6] VH1, a provascular cell-specific receptor kinase that influences leaf cell patterns in Arabidopsis [J].

Clay, NK ;

Nelson, T .

PLANT CELL, 2002, 14 (11) :2707-2722

[7] Arabidopsis SOMATIC EMBRYOGENESIS RECEPTOR KINASES1 and 2 are essential for tapetum development and microspore maturation [J].

Colcombet, J ;

Boisson-Dernier, A ;

Ros-Palau, R ;

Vera, CE ;

Schroeder, JI .

PLANT CELL, 2005, 17 (12) :3350-3361

[8] The CLAVATA1-related BAM1, BAM2 and BAM3 receptor kinase-like proteins are required for meristem function in Arabidopsis [J].

DeYoung, BJ ;

Bickle, KL ;

Schrage, KJ ;

Muskett, P ;

Patel, K ;

Clark, SE .

PLANT JOURNAL, 2006, 45 (01) :1-16

[9] A receptor kinase gene regulating symbiotic nodule development [J].

Endre, G ;

Kereszt, A ;

Kevei, Z ;

Mihacea, S ;

Kaló, P ;

Kiss, GB .

NATURE, 2002, 417 (6892) :962-966

[10] The 14-amino acid CLV3, CLE19, and CLE40 peptides trigger consumption of the root meristem in Arabidopsis through a CLAVATA2-dependent pathway [J].

Fiers, M ;

Golemiec, E ;

Xu, J ;

van der Geest, L ;

Heidstra, R ;

Stiekema, W ;

Liu, CM .

PLANT CELL, 2005, 17 (09) :2542-2553

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