Validation and application of a liquid chromatographic-mass spectrometric method for determination of artesunate in pharmaceutical samples

被引：30

作者：

Lindegardh, N. ^{[1
]}

Dondorp, A. M.

Singhasivanon, P.

White, N. J.

Day, N. P. J.

机构：

[1] Mahidol Univ, Fac Trop Med, Bangkok 10400, Thailand

[2] Univ Oxford, Nuffield Dept Clin Med, Ctr Trop Med, Oxford, England

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2007年 / 45卷 / 01期

基金：

英国惠康基金;

关键词：

MS; mass spectrometry; artesunate; determination; malaria; LC;

D O I：

10.1016/j.jpba.2007.04.030

中图分类号：

O65 [分析化学];

学科分类号：

070302 [分析化学]; 081704 [应用化学];

摘要：

A simple and rapid liquid chromatographic-mass spectrometric assay for the evaluation of artesunate in vials for injection has been developed and validated. The content of each vial was dissolved in 3.0 mL of methanol using a SGE analytical syringe (1.0 mL). Each sample was diluted to a theoretical concentration of 1000 ng/mL and analysed in triplicate. Three replicates of calibration standards at concentrations 500, 1000 and 1500 ng/mL were used to construct a calibration curve. Artesunate was analysed by liquid chromatography with atmospheric pressure chemical ionisation (APCI) mass spectrometric (MS) detection on a Hypersil Gold column (100 turn x 4.6 mm) using a mobile phase containing methanol-ammonium acetate 10 mM pH 5.3 (70:30, v/v) at a flow rate of 1 mL/min. The assay was implemented for the analysis of artesunate for injection purchased from Guilin Pharmaceutical Company in China. (C) 2007 Elsevier B.V. All rights reserved.

引用

页码：149 / 153

页数：5

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