The SH2 domain containing inositol 5-phosphatase SHIP2 displays phosphatidylinositol 3,4,5-trisphosphate and inositol 1,3,4,5-tetrakisphosphate 5-phosphatase activity

被引:99
作者
Pesesse, X
Moreau, C
Drayer, AL
Woscholski, R
Parker, P
Erneux, C
机构
[1] Free Univ Brussels, Interdisciplinary Res Inst, B-1070 Brussels, Belgium
[2] Imperial Canc Res Fund, Prot Phosphorylat Lab, London WC2A 3PX, England
[3] Univ London Imperial Coll Sci Technol & Med, Inst Biochem, London SW7 2AZ, England
关键词
phosphatidylinositol metabolism; signal transduction;
D O I
10.1016/S0014-5793(98)01255-1
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Distinct forms of inositol and phosphatidylinositol polyphosphate 5-phosphatases selectively remove the phosphate from the 5-position of the inositol ring from both soluble and lipid substrates, SHIP1 is the 145-kDa SH2 domain-containing inositol 5-phosphatase expressed in haematopoietic cells, SHIP2 is a related but distinct gene product. We report here that SHIP2 can be expressed in an active form both in Escherichia coli and in COS-7 cells, A truncated 103-kDa recombinant protein could be purified from bacteria that display both inositol 1,3,4,5-tetrakisphosphate (InsP(4)) and phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P-3) phosphatase activities. COS-7 cell lysates transfected with SHIP2 had increased PtdIns(3,4,5)P-3 phosphatase activity as compared to the vector alone. (C) 1998 Federation of European Biochemical Societies.
引用
收藏
页码:301 / 303
页数:3
相关论文
共 14 条
[1]   The 145-kDa protein induced to associate with Shc by multiple cytokines is an inositol tetraphosphate and phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase [J].
Damen, JE ;
Liu, L ;
Rosten, P ;
Humphries, RK ;
Jefferson, AB ;
Majerus, PW ;
Krystal, G .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (04) :1689-1693
[2]   Post-translational modification of human brain type I inositol-1,4,5-trisphosphate 5-phosphatase by farnesylation [J].
DeSmedt, F ;
Boom, A ;
Pesesse, X ;
Schiffmann, SN ;
Erneux, C .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (17) :10419-10424
[3]   The family of inositol and phosphatidylinositol polyphosphate 5-phosphatases [J].
Drayer, AL ;
Pesesse, X ;
DeSmedt, F ;
Communi, D ;
Moreau, C ;
Emeux, C .
BIOCHEMICAL SOCIETY TRANSACTIONS, 1996, 24 (04) :1001-1005
[4]   Cloning and expression of a human placenta inositol 1,3,4,5-tetrakisphosphate and phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase [J].
Drayer, AL ;
Pesesse, X ;
DeSmedt, F ;
Woscholski, R ;
Parker, P ;
Erneux, C .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1996, 225 (01) :243-249
[5]   SOLUBLE AND PARTICULATE INS(1,4,5)P3/INS(1,3,4,5)P4 5-PHOSPHATASE IN BOVINE BRAIN [J].
ERNEUX, C ;
LEMOS, M ;
VERJANS, B ;
VANDERHAEGHEN, P ;
DELVAUX, A ;
DUMONT, JE .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1989, 181 (02) :317-322
[6]   THE DEPHOSPHORYLATION PATHWAY OF D-MYO-INOSITOL 1,3,4,5-TETRAKISPHOSPHATE IN RAT-BRAIN [J].
ERNEUX, C ;
DELVAUX, A ;
MOREAU, C ;
DUMONT, JE .
BIOCHEMICAL JOURNAL, 1987, 247 (03) :635-639
[7]   Growth factors and insulin stimulate tyrosine phosphorylation of the 51C/SHIP2 protein [J].
Habib, T ;
Hejna, JA ;
Moses, RE ;
Decker, SJ .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (29) :18605-18609
[8]   Multiple forms of an inositol polyphosphate 5-phosphatase form signaling complexes with Shc and Grb2 [J].
Kavanaugh, WM ;
Pot, DA ;
Chin, SM ;
DeuterReinhard, M ;
Jefferson, AB ;
Norris, FA ;
Masiarz, FR ;
Cousens, LS ;
Majerus, PW ;
Williams, LT .
CURRENT BIOLOGY, 1996, 6 (04) :438-445
[9]   p150(Ship), a signal transduction molecule with inositol polyphosphate-5-phosphatase activity [J].
Lioubin, MN ;
Algate, PA ;
Tsai, S ;
Carlberg, K ;
Aebersold, R ;
Rohrschneider, LR .
GENES & DEVELOPMENT, 1996, 10 (09) :1084-1095
[10]   Inositols do it all [J].
Majerus, PW .
GENES & DEVELOPMENT, 1996, 10 (09) :1051-1053