Telomerase expression in gliomas including the nonastrocytic tumors

被引:34
作者
Chong, EYY
Lam, PYP
Poon, WS
Ng, HK
机构
[1] Chinese Univ Hong Kong, Dept Anat & Cellular Pathol, Hong Kong, Hong Kong
[2] Chinese Univ Hong Kong, Dept Surg, Neurosurg Unit, Hong Kong, Hong Kong
关键词
telomerase; gliomas; astrocytic; nonastrocytic;
D O I
10.1016/S0046-8177(98)80009-9
中图分类号
R36 [病理学];
学科分类号
100104 ;
摘要
Telomerase is a ribonucleoprotein that consists of a RNA component for sythesizing telomeric DNA repeats onto chromosome ends, so that telomere length can be maintained; telomerase activation in general signifies immortalization of cells. Because the telomerase activity of only a few cases of nonastrocytic gliomas and low-grade astrocytic gliomas have been examined before, telomerase activity from a broad spectrum of astrocytic and nonastrocytic gliomas were examined in this study. A total of 119 brain tumor samples, including 16 pilocytic astrocytomas, one dysembryoplastic neuroepithelial tumor (DNT), two pleomorphic xanthoastrocytomas (PXA), 15 ependymomas, 21 oligodendrogliomas, 13 grade II astrocytomas, 13 anaplastic astrocytomas, and 38 glioblastoma multiforme tumors (GBM), were studied, using a polymerase chain reaction (PCR)-based telomeric repeat amplification protocol assay. All pilocytic astrocytomas, DNT, PXA, and myxopapillary ependymomas were telomerase negative. Three of 13 (15.4%) classical ependymomas, 2 of 14 (14.3%) grade II oligodendrogliomas, and three of seven (42.9%) anaplastic oligodendrogliomas had detectable telomerase activity. The frequency of telomerase expression in ependymomas and oligodendrogliomas was lower than those observed in astrocytic tumors: fibrillary astrocytoma, 23.1% anaplastic astrocytoma, 23.1%; and GEM, 26.3%. The mean age of telomerase-positive GEM patients (61.7 years) was significantly higher than that of telomerase-negative GB,II patients (47.8 years, P=.002). These results suggest that telomerase activation may occur early in glial tumorigenesis, and astrocytomas may have mechanisms of immortalization other than telomerase activation. HUM PATHOL 29:599-603. Copyright (C) 1998 by W.B. Saunders Company.
引用
收藏
页码:599 / 603
页数:5
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