X-linked inhibitor of apoptosis protein expression and the regulation of apoptosis during human placental development

被引:44
作者
Gruslin, A
Qiu, Q
Tsang, BK
机构
[1] Ottawa Hosp, Div Maternal Fetal Med, Dept Obstet Gynecol & Newborn Care, Loeb Res Inst, Ottawa, ON K1H 8L6, Canada
[2] Univ Ottawa, Div Maternal Fetal Med, Reprod Biol Unit, Ottawa, ON K1H 8L6, Canada
[3] Univ Ottawa, Div Reprod Med, Dept Obstet & Gynecol, Ottawa, ON K1H 8L6, Canada
关键词
apoptosis; developmental biology; placenta; pregnancy; trophoblast;
D O I
10.1095/biolreprod64.4.1264
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
In this study, we have examined the expression and potential role of X-linked inhibitor of apoptosis protein (XIAP), Pas, and Fas ligand (FasL) in the regulation of apoptosis throughout placental development. Protein expression was determined by Western blot analysis and immunohistochemistry, whereas apoptotic cell death was assessed by DNA fragmentation analysis and TUNEL. The XIAP was present in trophoblast throughout placental development, but its content significantly decreased during late pregnancy, when apoptosis was maximal. The FasL content was low during early placental development but increased coincidentally to the decrease in XIAP during the third trimester. Our data also suggest that placental apoptosis is the culmination of the relative expression of these cell-death and -survival proteins, a phenomenon that is cell type-specific and dependent on cytodifferentiation and the stage of placental development. Moreover, the induction of syncytiotrophoblast apoptosis may involve the concomitant up-regulation of FasL for Pas activation and the removal of downstream inhibition of the apoptotic cascade by XIAP.
引用
收藏
页码:1264 / 1272
页数:9
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