Novel method for rapid measurement of growth of mycobacteria in detergent-free media

被引：46

作者：

Meyers, PR

Bourn, WR

Steyn, LM

Van Helden, P

Beyers, AD

Brown, GD

机构：

[1] Univ Stellenbosch, Dept Med Biochem, MRC Ctr Mol & Cellular Biol, ZA-7505 Tygerberg, South Africa

[2] Univ Cape Town, Dept Med Microbiol, Observ, ZA-7925 Cape Town, South Africa

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1998年 / 36卷 / 09期

基金：

英国惠康基金;

关键词：

D O I：

10.1128/JCM.36.9.2752-2754.1998

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We describe a novel, rapid, and inexpensive method for the measurement of growth of Mycobacterium tuberculosis, Mycobacterium bovis, and Mycobacterium smegmatis in the presence or absence of detergent. The method, which employs hot NaOH treatment of mycobacterial cells to release total cellular protein, compares favorably with other methods for monitoring mycobacterial growth but is particularly useful for heavily clumped cultures grown in defined minimal medium.

引用

页码：2752 / 2754

页数：3

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