High-throughput plasmid DNA purification for 3 cents per sample

被引：40

作者：

Marra, Marco A. ^{[1
]}

Kucaba, Tamara A. ^{[1
]}

Hillier, LaDeana W. ^{[1
]}

Waterston, Robert H. ^{[1
]}

机构：

[1] Washington Univ, Genome Sequencing Ctr, St Louis, MO 63108 USA

来源：

NUCLEIC ACIDS RESEARCH | 1999年 / 27卷 / 24期

关键词：

D O I：

10.1093/nar/27.24.e37

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To accommodate the increasingly rapid rates of DNA sequencing we have developed and implemented an inexpensive, expeditious method for the purification of double-stranded plasmid DNA clones. The robust nature, high throughput, low degree of technical difficulty and extremely low cost have made it the plasmid DNA preparation method of choice in both our expressed sequence tag (EST) and genome sequencing projects. Here we report the details of the method and describe its application in the generation of more than 700 000 ESTs at a rate exceeding 16 000 per week.

引用

页数：6

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