Synthesis of oligosaccharides catalyzed by thermostable β-glucosidase from Pyrococcus furiosus

被引:17
作者
Boon, MA
van der Oost, J
de Vos, WM
Janssen, AEM
Van't Riet, K
机构
[1] Wageningen Univ Agr, Food & Bioproc Engn Grp, NL-6700 EV Wageningen, Netherlands
[2] Wageningen Univ Agr, Dept Microbiol, NL-6703 CT Wageningen, Netherlands
关键词
Pyrococcus furiosus; beta-glucosidase; thermostable enzyme; oligosaccharide synthesis;
D O I
10.1007/BF02787780
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The thermostable beta-glucosidase from Pyrococcus furiosus has been shown to produce tri- and tetrasaccharides with lactose as a substrate (0.73-1.44 mol/kg) at elevated temperatures (75-95 degrees C). The enzyme has a broad pH optimum (5.0-7.0), and was inhibited more by glucose than by galactose. An increase in the initial lactose concentrations led to a proportional increase of the ratio between the initial oligosaccharide production rate and the initial lactose conversion rate. Because of inhibition by Maillard components, the lowest temperature studied (75 degrees C) is the best for oligosaccharide synthesis. The oligosaccharide yield obtained with this thermostable enzyme is independent of the initial lactose concentration and temperature and a factor 1.4 higher than reported for mesophilic enzymes.
引用
收藏
页码:269 / 278
页数:10
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