A HAPPY map of Cryptosporidium parvum

被引：48

作者：

Piper, MB ^{[1
]}

Bankier, AT ^{[1
]}

Dear, PH ^{[1
]}

机构：

[1] MRC, Mol Biol Lab, Prot & Nucl Acid Chem Div, Cambridge CB2 2QH, England

来源：

GENOME RESEARCH | 1998年 / 8卷 / 12期

关键词：

D O I：

10.1101/gr.8.12.1299

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have constructed a HAPPY map of the apicomplexan parasite Cryptosporidium parvum. We have placed 204 markers on the 10.4-Mb genome, giving an average marker spacing of similar to 50 kb, with an effective resolution of similar to 40 kb. HAPPY mapping (an in vitro linkage technique based on screening approximately <(hap)under bar>loid amounts of DNA by the (p) under bar ol (y) under bar merase chain reaction) is fast and accurate and is not subject to the distortions inherent in cloning, meiotic recombination, or hybrid cell formation. In addition, little genomic DNA is needed as a substrate, and the AT content of the genome is largely immaterial, making it an ideal method for mapping otherwise intractable parasite genomes. The map, covering all eight chromosomes, consists of 10 linkage groups, each of which has been chromosomally assigned. We have verified the accuracy of the map by several methods, including the construction of a >140-kb PAC contig on chromosome VI. Less than 1% of our markers detect non-rDNA duplicated sequences.

引用

页码：1299 / 1307

页数：9

共 37 条

[1]

BANKIER AT, 1987, METHOD ENZYMOL, V155, P51

[2]

BANKIER AT, 1989, NUCLEIC ACID SEQUENC, P37

[3] Molecular karyotype analysis of Cryptosporidium parvum: Evidence for eight chromosomes and a low-molecular-size molecule [J].

Blunt, DS ;

Khramtsov, NV ;

Upton, SJ ;

Montelone, BA .

CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY, 1997, 4 (01) :11-13

[4]

Bonnin A, 1996, FEMS MICROBIOL LETT, V137, P207, DOI 10.1016/0378-1097(96)00056-0

[5] The beta-tubulin gene of Cryptosporidium parvum [J].

Caccio, S ;

LaRosa, G ;

Pozio, E .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1997, 89 (02) :307-311

[6]

CACCIO S, 1998, GENE

[7] RADIATION HYBRID MAPPING - A SOMATIC-CELL GENETIC METHOD FOR CONSTRUCTING HIGH-RESOLUTION MAPS OF MAMMALIAN CHROMOSOMES [J].

COX, DR ;

BURMEISTER, M ;

PRICE, ER ;

KIM, S ;

MYERS, RM .

SCIENCE, 1990, 250 (4978) :245-250

[8] A high-resolution metric HAPPY map of human chromosome 14 [J].

Dear, PH ;

Bankier, AT ;

Piper, MB .

GENOMICS, 1998, 48 (02) :232-241

[9] HAPPY MAPPING - LINKAGE MAPPING USING A PHYSICAL ANALOG OF MEIOSIS [J].

DEAR, PH ;

COOK, PR .

NUCLEIC ACIDS RESEARCH, 1993, 21 (01) :13-20

[10]

DEAR PH, 1997, GENOME MAPPING PRACT, P95

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