Selection of DNA aptamers against VEGF165 using a protein competitor and the aptamer blotting method

被引：76

作者：

Hasegawa, Hijiri ^{[1
]}

Sode, Koji ^{[1
]}

Ikebukuro, Kazunori ^{[1
]}

机构：

[1] Tokyo Univ Agr & Technol, Dept Biotechnol & Life Sci, Koganei, Tokyo 1848588, Japan

来源：

BIOTECHNOLOGY LETTERS | 2008年 / 30卷 / 05期

关键词：

aptamer blotting; cancer diagnosis; competitor; DNA aptamer; sensor element; VEGF165;

D O I：

10.1007/s10529-007-9629-6

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Two DNA aptamers against a tumor marker protein, human vascular endothelial growth factor (VEGF(165)) were identified. In the screening process, another protein was used as the competitor to isolate those aptamers that have high specificity for the target. In addition, we evaluated the affinities of the enriched library by means of aptamer blotting. The isolated aptamers bound to VEGF(165) with a K (d) value in the range of a few hundred nanomoles, and did not bind to the competitor. This selection method enabled us to efficiently select the specific aptamers against the target protein. These specific aptamers would be useful sensor elements for cancer diagnosis.

引用

页码：829 / 834

页数：6

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